QTBL of OMICs 2nd Week

Base-pairing occurs between the  tRNA and the mRNA to ensure that each amino acid is inserted into the growing polypeptide at the appropriate position. Please choose the correct option regarding this process

•    The codon in the mRNA is pairing with the anticodon in the tRNA , for example AUG in mRNA with amino acid methionine-tRNA complex.

•   The codon in the mRNA is pairing with the anticodon in the tRNA , for example UAG in mRNA with amino acid methionine-tRNA complex.

•  The codon in the mRNA is pairing with the anticodon in the tRNA , for example UAG in mRNA with amino acid methionine-tRNA complex.

• The anti codon in the mRNA is pairing with the codon in the tRNA , for example UGA in mRNA with amino acid methionine-tRNA complex.

• The anti codon in the mRNA is pairing with the codon in the tRNA , for example AUG  in mRNA with amino acid methionine-tRNA complex.

Bayu is learning the processes of DNA transcription. He is fascinated by the fact that there is a specific flow of the copying process of DNA to the primary RNA. The correct statement about the flow is…

• The DNA coding chain is copied in the 3’to 5’ direction, and the RNA transcript is synthesized in the 5; to 3’ direction.

• The DNA template chain is copied in the 3’to 5’ direction, and the RNA transcript is synthesized in the 5’ to 3’ direction.

• The DNA coding chain is copied in the 5’to 3’ direction, and the RNA transcript is synthesized in the 3’ to 5’ direction.

• The DNA template chain is copied in the 5’to 3’ direction, and the RNA transcript is synthesized in the 3’ to 5’ direction.

• The DNA coding chain is copied in the 5’to 3’ direction, and the primary RNA  is synthesized in the 5’ to 3’ direction.

After reading a chapter about DNA transcription in a  biochemistry textbook, Bayu recognizes that there are some types of RNA, which are , hn RNA, mRNA, t-RNA, micro RNA. There are some differences between hn RNA and mRNA. Choose the correct statement about these differences.

•     Both the hn RNA and mRNA has a guanosine “cap” added at the 5’ end, a poly(A) “tail” at the 3’-end, exon, and intron.

• The hn mRNA has intron and exon, while the mRNA has a guanosine “cap” added at the 5’-end, a poly(A) “tail” at the 3’-end, and exon.

• The mRNA has both exons and introns, hn RNA has exon only

• The mRNA has both exons and introns, hn RNA has intron only

• The mRNA has intron, hn RNA has exon and intron.

In order to grow, baby’s cells are duplicating rapidly, through mitosis. In the G1 phase, the proteins are synthesized to prepare for the DNA replication in S phase. Energy is highly needed during this phase. In transcription, the energy is needed to pair a nucleotide base at the DNA template strand to a nucleotide triphosphate and to continue the polymerization during mRNA chain formation. The energy is from:

•   cleavage of a phosphate bond in the nucleotide triphosphate and release of pyrophosphate, as well as the cleavage of the pyrophosphate by a pyrophosphatase

• cleavage of oxygen from bond in the nucleoside triphosphate and release of pyrophosphate, as well as the cleavage of the pyrophosphate by a pyrophosphatase

•  the cleavage of the nucleotide triphosphate by a pyrophosphatase

• he cleavage of the nucleoside triphosphate by a pyrophosphatase

•   the cleavage of ADP into AMP

Beta thalassemia can be caused by one of the two mutations in a region upstream, i.e. in the region between -20 to -30.  In this region, that is rich with nucleotide base Adenine or Thymine, there is a point mutation that changes nucleotide base adenine to cytosine or guanine. What is the name of this region?

• enhancer

• promotor

• proximal – promotor element

• TATA box

• CAAT box.

The mode of inheritance of Marfan syndrome in the pedigree below is:

•  Autosomal dominant

• Autosomal recessive

• X-linked dominant

• X-linked recessive

• Mitochondria

Dona is studying about central dogma. She has completely read two chapters about DNA transcription and RNA  translation. In a segment of a transcribed gene, the non template strand of DNA has the following sequence: 5’...AGCTCACTG...3’. What will be the corresponding sequence in the RNA produced from this segment of the gene?

•  CAGUGAGCU

•    AGCUCACUG

• CAGTGAGCT

• UCGAGUGAC

•  GTCACTCGA