Lab Science - Aseptic Techniques

What is the main purpose of using aseptic techniques in a laboratory setting?

• Increase growth rate of bacteria

• Prevent contamination of samples

• Enhance the colour of cultures

• Reduce the cost of experiments

What temperature does the agar need to be at prior to pouring?

• -20 ⁰C

• 5 ⁰C

• 25 ⁰C

• 50 ⁰C

Tags

NGSS.MS-PS1-4

When transferring liquid culture to another liquid medium, which tool is commonly used?

• Scalpel

• micropipette

• Petri dish

• Microscope slide

Match the equipment to their name.

• micropipette

• 
• bunsen burner

• 
• goggles

• 
• glass spreader

• 
• petri dish

• 

What do you need to do prior to performing an aseptic technique?

It is important to ​ (a)   the ​ (b)   of a bottle ​ (c)   and after ​ (d)   cultures in order to avoid​ (e)   of samples.

• flame
• neck
• before
• transferring
• contamination
• discarding
• boil
• shake
• freeze
• after

Reorder the following steps for pouring an agar plate.

• Replace slide and leave to solidify

• Carefully pour agar until surface is covered

• Remove lid slightly

• Allow to rest of 5 minutes

• Label petri dish on underside

Label the underside of this petri dish correctly.

a

b

c

Initials

Date made

Microorganism cultured

How should you calculate the magnification of a specimen viewed under a microscope?

• eyepiece magnification x objective lens magnification

• eyepiece magnification + objective lens magnification

• eyepiece magnification ÷ objective lens magnification

• eyepiece magnification -objective lens magnification

What is the total magnification of a microscope with a 10x eyepiece and a 40x objective lens?

• 400x

• 50x

• 100x

• 4x