1.1 Laboratory Techniques Quiz

What is the purpose of a risk assessment in a laboratory setting?

• To eliminate all hazards

• To identify control measures to minimize risk

• To determine the cost of lab materials

• To prioritize which experiments to conduct first

What type of dilution involves equal intervals between concentrations, such as 0.1, 0.2, 0.3?

• Exponential dilution

• Log dilution

• Serial dilution

• Linear dilution

Which laboratory technique is used to separate substances based on their density?

• Paper chromatography

• Gel electrophoresis

• Centrifugation

• Affinity chromatography

What is the role of a buffer in a solution?

• To increase the pH rapidly

• To maintain a constant pH

• To enhance the color change of a solution

• To act as a solvent for dilutions

How does SDS-PAGE separate proteins?

• By size, shape, and charge

• By their isoelectric points only

• By size alone

• By density

Which technique uses specific antibodies to detect the presence of proteins?

• Western blotting

• Centrifugation

• Fluorescence microscopy

• Thin layer chromatography

What type of microscopy involves the use of fluorescent labels to visualize specific molecules?

• Bright-field microscopy

• Electron microscopy

• Phase-contrast microscopy

• Fluorescence microscopy

What is the purpose of aseptic technique in a laboratory?

• To increase cell growth speed

• To sterilize all glassware permanently

• To eliminate unwanted microbial contaminants

• To optimize nutrient availability for microbes

What is the key characteristic of a standard curve in determining unknown concentrations?

• It measures pH changes.

• It relates the number of viable cells to a culture.

• It uses known concentrations to estimate unknown ones.

• It separates proteins by charge.

What is the function of growth factors in cell culture?

• To maintain a sterile environment

• To measure the pH of the culture

• To promote cell growth and proliferation

• To separate proteins from cells