Enzyme Practical Quiz

What is the aim of the enzyme required practical?

• To investigate the effect of light on the rate of reaction of amylase

• To investigate the effect of concentration on the rate of reaction of amylase

• To investigate the effect of pH on the rate of reaction of amylase

• To investigate the effect of temperature on the rate of reaction of amylase

What enzyme is used in the practical to break down starch?

• Lipase

• Protease

• Amylase

• Catalase

What is used as an indicator for the reaction occurring in the enzyme practical?

• Ethanol

• Iodine solution

• Biuret solution

• Benedict's solution

What color does iodine turn in the presence of starch?

• Red

• Blue-black

• Green

• Yellow

What is the purpose of using a pH buffer solution in the experiment?

• To change the temperature

• To increase the reaction rate

• To maintain a constant pH

• To decrease the reaction rate

How often should you place a drop of the mixture on iodine during the experiment?

• Every 5 seconds

• Every 10 seconds

• Every 15 seconds

• Every 20 seconds

What does it indicate if the iodine solution remains orange-brown?

• All starch has been digested

• The pH is too high

• The enzyme is inactive

• Starch is still present

What can be used to control the temperature during the experiment?

• A colorimeter

• A thermometer

• A water bath

• A pH meter

What is the role of a colorimeter in this experiment?

• To measure light absorbance

• To measure temperature

• To measure pH

• To measure enzyme concentration

What is the substrate in the enzyme practical?

• Glucose

• Starch

• Maltose

• Protein