Which phase of the Pentose Phosphate Pathway generates NADPH?
9.5 Microbial Physiology Module 9: Metabolism

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Biology
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Professional Development
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Hard
MicroCore RC
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13 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Oxidative phase
Non-oxidative phase
Both phases
Neither phase
Answer explanation
Answer: a. NADPH is produced during the oxidative phase from glucose-6-phosphate
References:
Brock et al., 2021
Struck & White, 2019
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which enzyme catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolactone?
Glucose-6-phosphate dehydrogenase
Phosphofructokinase
Hexokinase
Transaldolase
Answer explanation
Answer: a. This step initiates the oxidative phase of the PPP and generates NADPH
References:
Brock et al., 2021
Reyes, J.S., Fuentes-Lemus, E., Figueroa, J.D. et al. Implications of differential peroxyl radical-induced inactivation of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase for the pentose phosphate pathway. Sci Rep 12, 21191 (2022). https://doi.org/10.1038/s41598-022-25474-x
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which molecule is a key product of the non-oxidative phase of the PPP?
Fructose-6-phosphate
Glucose
Pyruvate
NADH
Answer explanation
Answer: a. The non-oxidative phase produces intermediates like fructose-6-phosphate and glyceraldehyde-3-phosphate for glycolysis
References:
Brock et al., 2021
Gupta, R., Gupta, N. (2021). Pentose Phosphate Pathway. In: Fundamentals of Bacterial Physiology and Metabolism. Springer, Singapore. https://doi.org/10.1007/978-981-16-0723-3_10
Struck & White, 2019
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which key intermediate is formed during the Entner-Doudoroff pathway?
Glucose-6-phosphate
6-phosphogluconate
Fructose-1,6-bisphosphate
Succinyl-CoA
Answer explanation
Answer: b
Rationale: 6-phosphogluconate is an intermediate specific to the Entner-Doudoroff pathway
References:
Brock et al., 2021
Spector, M. (2009). Metabolism, central (Intermediary). In Elsevier eBooks (pp. 242–264). https://doi.org/10.1016/b978-012373944-5.00078-x
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the main product of the Entner-Doudoroff pathway?
Glucose
2 ATP
NADPH and pyruvate
Acetyl-CoA
Answer explanation
Answer: c
Rationale: This pathway generates pyruvate and NADPH, important for metabolism and biosynthesis
References:
Brock et al., 2021
Spector, M. (2009). Metabolism, central (Intermediary). In Elsevier eBooks (pp. 242–264). https://doi.org/10.1016/b978-012373944-5.00078-x
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the primary function of the Methylglyoxal pathway?
Degradation of glucose to CO₂
Detoxification of methylglyoxal
ATP production
Lipid synthesis
Answer explanation
Answer: b. The pathway helps detoxify methylglyoxal, a toxic intermediate formed during glycolysis
References:
Kammerscheit X, Hecker A, Rouhier N, Chauvat F, Cassier-Chauvat C.2020.Methylglyoxal Detoxification Revisited: Role of Glutathione Transferase in Model Cyanobacterium Synechocystis sp. Strain PCC 6803. mBio11:10.1128/mbio.00882-20.https://doi.org/10.1128/mbio.00882-20
Struck & White, 2019
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which enzyme converts methylglyoxal to lactate?
Lactate dehydrogenase
Glyoxalase I
Aldolase
Glyoxalase II
Answer explanation
Answer: d. Glyoxalase II converts methylglyoxal intermediates to lactate, detoxifying the compound
References:
Brock et al., 2021
Kammerscheit, X., Hecker, A., Rouhier, N., Chauvat, F., & Cassier-Chauvat, C. (2020). Methylglyoxal Detoxification Revisited: Role of Glutathione Transferase in Model Cyanobacterium Synechocystis sp. Strain PCC 6803. mBio, 11(4). https://doi.org/10.1128/mbio.00882-20
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