9.5 Microbial Physiology Module 9: Metabolism

9.5 Microbial Physiology Module 9: Metabolism

Professional Development

13 Qs

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9.5 Microbial Physiology Module 9: Metabolism

9.5 Microbial Physiology Module 9: Metabolism

Assessment

Quiz

Biology

Professional Development

Hard

Created by

MicroCore RC

Used 4+ times

FREE Resource

13 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which phase of the Pentose Phosphate Pathway generates NADPH?

Oxidative phase

Non-oxidative phase

Both phases

Neither phase

Answer explanation

Media Image

Answer: a. NADPH is produced during the oxidative phase from glucose-6-phosphate

References:

Brock et al., 2021

Struck & White, 2019

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which enzyme catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolactone?

Glucose-6-phosphate dehydrogenase

Phosphofructokinase

Hexokinase

Transaldolase

Answer explanation

Media Image

Answer: a. This step initiates the oxidative phase of the PPP and generates NADPH

References:

Brock et al., 2021

Reyes, J.S., Fuentes-Lemus, E., Figueroa, J.D. et al. Implications of differential peroxyl radical-induced inactivation of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase for the pentose phosphate pathway. Sci Rep 12, 21191 (2022). https://doi.org/10.1038/s41598-022-25474-x

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which molecule is a key product of the non-oxidative phase of the PPP?

Fructose-6-phosphate

Glucose

Pyruvate

NADH

Answer explanation

Media Image

Answer: a. The non-oxidative phase produces intermediates like fructose-6-phosphate and glyceraldehyde-3-phosphate for glycolysis

References:

Brock et al., 2021

Gupta, R., Gupta, N. (2021). Pentose Phosphate Pathway. In: Fundamentals of Bacterial Physiology and Metabolism. Springer, Singapore. https://doi.org/10.1007/978-981-16-0723-3_10

Struck & White, 2019

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which key intermediate is formed during the Entner-Doudoroff pathway?

Glucose-6-phosphate

6-phosphogluconate

Fructose-1,6-bisphosphate

Succinyl-CoA

Answer explanation

Media Image

Answer: b

Rationale: 6-phosphogluconate is an intermediate specific to the Entner-Doudoroff pathway

References:

Brock et al., 2021

Spector, M. (2009). Metabolism, central (Intermediary). In Elsevier eBooks (pp. 242–264). https://doi.org/10.1016/b978-012373944-5.00078-x

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the main product of the Entner-Doudoroff pathway?

Glucose

2 ATP

NADPH and pyruvate

Acetyl-CoA

Answer explanation

Media Image

Answer: c

Rationale: This pathway generates pyruvate and NADPH, important for metabolism and biosynthesis

References:

Brock et al., 2021

Spector, M. (2009). Metabolism, central (Intermediary). In Elsevier eBooks (pp. 242–264). https://doi.org/10.1016/b978-012373944-5.00078-x

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the primary function of the Methylglyoxal pathway?

Degradation of glucose to CO₂

Detoxification of methylglyoxal

ATP production

Lipid synthesis

Answer explanation

Media Image

Answer: b. The pathway helps detoxify methylglyoxal, a toxic intermediate formed during glycolysis

References:

Kammerscheit X, Hecker A, Rouhier N, Chauvat F, Cassier-Chauvat C.2020.Methylglyoxal Detoxification Revisited: Role of Glutathione Transferase in Model Cyanobacterium Synechocystis sp. Strain PCC 6803. mBio11:10.1128/mbio.00882-20.https://doi.org/10.1128/mbio.00882-20

Struck & White, 2019

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which enzyme converts methylglyoxal to lactate?

Lactate dehydrogenase

Glyoxalase I

 Aldolase

Glyoxalase II

Answer explanation

Media Image

Answer: d. Glyoxalase II converts methylglyoxal intermediates to lactate, detoxifying the compound

References:

Brock et al., 2021

Kammerscheit, X., Hecker, A., Rouhier, N., Chauvat, F., & Cassier-Chauvat, C. (2020). Methylglyoxal Detoxification Revisited: Role of Glutathione Transferase in Model Cyanobacterium Synechocystis sp. Strain PCC 6803. mBio, 11(4). https://doi.org/10.1128/mbio.00882-20

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