RestrictionEnzymes

What is the primary function of restriction enzymes in bacterial cells?

• • Replication of DNA

• • Protection against viral DNA

• • DNA synthesis

• • Transcription of DNA

Which enzyme adds an adenine (A) overhang to the 3' ends of PCR products?

• DNA Ligase

• Taq DNA Polymerase

• EcoRI

• RNA Polymerase

What discovery in the 1960s led to the characterization of the host-controlled restriction and modification system?

• The discovery of CRISPR

• The observation of restricted growth of bacteriophage λ in E. coli strains

• The invention of PCR

• The isolation of EcoRI

HindII, was isolated from which organism?

• Escherichia coli

• Haemophilus influenzae

• Saccharomyces cerevisiae

• Streptococcus pneumoniae

The first type II enzyme isolated was