Methods in Gene Cloning

Which of the followings is NOT a step that involved in gene cloning?

• Screen the bacterial cell that contain recombinant DNA.

• Insert DNA fragments into open plasmid and join them using DNA ligase.

• Cut the plasmid and target DNA with modifying enzyme.

• Isolate the plasmid from bacterial cell and target DNA from donor cell.

What is the purpose of screening?

• to identify host cell containing plasmid

• to identify host cell that able to produce β-galactosidase

• to identify host cell containing the recombinant DNA

• to identify host cell with DNA

Which of these pairs is INCORRECTLY matched?

• DNA ligase - mapping human chromosome

• Target DNA - gene of interest

• Taq polymerase - PCR

• Plasmid as vector - isolated from bacteria

Marker gene that encodes for resistant to ampicillin is amp^R. What will happen if the plasmid lack of this gene.

• Transformed bacteria can be separated from non transformed bacteria.

• Non-transformed bacteria cannot be separated from transformed bacteria.

• Transformed bacteria are able to survive in antibiotic ampicillin.

• Transformed bacteria are able to form white colony.

What is the purpose of amplification?

• To screen for recombinant plasmid

• To join the recombinant DNA with the host cell

• To produce multiple copies of the desired gene

• To prevent the bacteria from reproducing

During the amplification of target gene, an antibiotic medium used to

• select for plasmids containing particular DNA fragments

• select against plasmids containing human DNA fragments

• select for bacteria lacking plasmids

• select for bacteria containing plasmids

Which of the following is the CORRECT sequence in gene cloning?

• Screening → Isolation → Cut → Insertion → Transformation and amplification

• Isolation → Cut → Insertion → Screening → Transformation and amplification

• Screening → Transformation and amplification → Cut → Insertion → Isolation

• Isolation → Cut → Insertion → Transformation and amplification → Screening

What is transformation in recombinant DNA technology?

• Synthesis of mRNA from a DNA molecule

• Insertion of mRNA into host cell

• Insertion of recombinant DNA into host cell

• Synthesis of DNA molecule using mRNA template

Which genetic marker will be altered if a plasmid accepted a gene of interest during cloning process?

• lacY gene

• lacA gene

• lacZ gene

• Ampicilin resistance gene

In order to insert a human gene into plasmid, both must

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.