Genetics PASS Quiz Buster 3, 2024

Which statement about the polymerase chain reaction process is correct?

• Denaturation: 95°C, Annealing: 50-56°C, Extension: 72°C

• Denaturation: 50-56°C, Annealing: 72°C, Extension: 95°C

• Denaturation: 72°C, Annealing: 50-56°C, Extension: 95°C

• Denaturation: 95°C, Annealing: 50-56°C, Extension: 95°C

Which sequence is palindromic?

• 5' ACGGATTCGC 3'

• 5' ATG 3'

• 5' AGGCCT 3'

• 5' TAGAT 3'

The restriction site for an enzyme called EcoRI is the following sequence:

5’ - G*A-A-T-T-C - 3’ 3’ - C-T-T-A-A*G - 5’

Staggered cuts are made between G and A on each strand. What type of bonds are being cleaved and what type of ends does EcoRI generate?

• Hydrogen bonds; cohesive ends with 5’ overhang

• Phosphodiester bonds; cohesive ends with 5’ overhang

• Phosphodiester bonds; cohesive ends with 3’ overhang

• Hydrogen bonds; blunt ends

The standard bacteria used in the production of recombinant DNA is:

• S. pneumoniae

• E. coli

• S. aureus

• M. tuberculosis

Protein Acrylamide Gel Electrophoresis (PAGE) uses all the following components EXCEPT:

• Reducing agent

• Detergent

• Pressure

• Stain

• Heat

Which blotting type is matched with the correct probe molecule?

• Western blotting – DNA

• Southern blotting – Antibody

• Northern blotting – Protein

• Western blotting – Antibody

The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily based on what factor?

• The size of the wells in the gel.

• The volume of the DNA sample loaded

• The positive charge of the DNA

• The size of the DNA fragments

Use the image to answer the following question: A segment of DNA has two restriction sites - I and II. When incubated with restriction enzymes I and II, three fragments will be formed - a, b and c.  Which of the following gels produced by electrophoresis would represent the separation and identity of these fragments?

• A

• B

• C

• D

How does manual sequencing differ from automated sequencing?

• Manual sequencing requires ddNTPs, whereas automated sequencing only requires dNTPs

• Automated sequencing involves four parallel polymerase reactions, whereas manual sequencing occurs in only one tube 

• The results of manual sequencing are captured by an autoradiogram of the electrophoresis gel, whereas the results of automated sequencing are captured by a fluorescence detector 

• Manual sequencing uses DNA polymerase, whereas automated sequencing utilises reverse transcriptase 

Which of the following is NOT a component in next generation DNA sequencing?

• Fragmented genomic DNA

• DNA polymerase

• Antibody probes

• dNTPs