Genetics PASS Quiz Buster 3, 2024

Genetics PASS Quiz Buster 3, 2024

University

20 Qs

quiz-placeholder

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Genetics PASS Quiz Buster 3, 2024

Genetics PASS Quiz Buster 3, 2024

Assessment

Quiz

Biology

University

Medium

Created by

Katie Grey

Used 3+ times

FREE Resource

20 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which statement about the polymerase chain reaction process is correct?

Denaturation: 95°C, Annealing: 50-56°C, Extension: 72°C

Denaturation: 50-56°C, Annealing: 72°C, Extension: 95°C

Denaturation: 72°C, Annealing: 50-56°C, Extension: 95°C

Denaturation: 95°C, Annealing: 50-56°C, Extension: 95°C

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which sequence is palindromic?

5' ACGGATTCGC 3'

5' ATG 3'

5' AGGCCT 3'

5' TAGAT 3'

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The restriction site for an enzyme called EcoRI is the following sequence:

5’ - G*A-A-T-T-C - 3’ 3’ - C-T-T-A-A*G - 5’

Staggered cuts are made between G and A on each strand. What type of bonds are being cleaved and what type of ends does EcoRI generate?

Hydrogen bonds; cohesive ends with 5’ overhang

Phosphodiester bonds; cohesive ends with 5’ overhang

Phosphodiester bonds; cohesive ends with 3’ overhang

Hydrogen bonds; blunt ends

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The standard bacteria used in the production of recombinant DNA is:

S. pneumoniae

E. coli

S. aureus

M. tuberculosis

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Protein Acrylamide Gel Electrophoresis (PAGE) uses all the following components EXCEPT:

Reducing agent

Detergent

Pressure

Stain

Heat

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which blotting type is matched with the correct probe molecule?

Western blotting – DNA

Southern blotting – Antibody

Northern blotting – Protein

Western blotting – Antibody

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily based on what factor?

The size of the wells in the gel.

The volume of the DNA sample loaded

The positive charge of the DNA

The size of the DNA fragments

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