Which statement about the polymerase chain reaction process is correct?

Genetics PASS Quiz Buster 3, 2024

Quiz
•
Biology
•
University
•
Medium

Katie Grey
Used 3+ times
FREE Resource
20 questions
Show all answers
1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Denaturation: 95°C, Annealing: 50-56°C, Extension: 72°C
Denaturation: 50-56°C, Annealing: 72°C, Extension: 95°C
Denaturation: 72°C, Annealing: 50-56°C, Extension: 95°C
Denaturation: 95°C, Annealing: 50-56°C, Extension: 95°C
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which sequence is palindromic?
5' ACGGATTCGC 3'
5' ATG 3'
5' AGGCCT 3'
5' TAGAT 3'
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
The restriction site for an enzyme called EcoRI is the following sequence:
5’ - G*A-A-T-T-C - 3’ 3’ - C-T-T-A-A*G - 5’
Staggered cuts are made between G and A on each strand. What type of bonds are being cleaved and what type of ends does EcoRI generate?
Hydrogen bonds; cohesive ends with 5’ overhang
Phosphodiester bonds; cohesive ends with 5’ overhang
Phosphodiester bonds; cohesive ends with 3’ overhang
Hydrogen bonds; blunt ends
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
The standard bacteria used in the production of recombinant DNA is:
S. pneumoniae
E. coli
S. aureus
M. tuberculosis
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Protein Acrylamide Gel Electrophoresis (PAGE) uses all the following components EXCEPT:
Reducing agent
Detergent
Pressure
Stain
Heat
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which blotting type is matched with the correct probe molecule?
Western blotting – DNA
Southern blotting – Antibody
Northern blotting – Protein
Western blotting – Antibody
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily based on what factor?
The size of the wells in the gel.
The volume of the DNA sample loaded
The positive charge of the DNA
The size of the DNA fragments
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