DNA Sequencing Quiz

What is the first step in DNA sequencing?

• Make copies of the DNA

• Obtain and isolate the DNA

• Make labeled fragments of the DNA

• Separate the fragments of DNA

What is done after separating the fragments of DNA?

• Obtain and isolate the DNA

• Make copies of the DNA

• Make labeled fragments of the DNA

• DNA sequence is analyzed

What is the first step in obtaining and isolating DNA according to the provided material?

• Lyse the cells in the sample

• Centrifuge the sample

• Get a sample

• Collect the supernatant using a micropipette

What tool is used to collect the supernatant in the DNA isolation process?

• Centrifuge

• Micropipette

• Scalpel

• Beaker

What is the final step in the DNA isolation process?

• Get a sample

• Lyse the cells in the sample

• Centrifuge the sample

• Collect the supernatant using a micropipette

What happens to the cell debris during the DNA isolation process?

• It is treated with protease

• It is lysed using a detergent

• It is pelleted in a centrifuge

• It is transferred to a clean tube

What is the main purpose of using protease in the DNA isolation process?

• To destroy RNA

• To destroy proteins

• To disrupt the plasma membrane

• To pellet cell debris

Which step directly follows the treatment of cell contents with protease and RNAase in the DNA isolation process?

• Lysing cells using a detergent

• Centrifuging cell debris

• Transferring the supernatant to a clean tube

• Disrupting the plasma membrane

Which device is used in the PCR process to produce millions of copies of DNA?

• Thermal cycler

• DNA sequencer

• Centrifuge

• Electrophoresis machine

What is the next step after making copies of DNA using PCR?

• The next slide shows the steps of PCR

• Sequencing the DNA

• Analyzing the DNA

• Storing the DNA