LTEE

The E. coli long-term evolution experiment (LTEE) is an ongoing study in experimental evolution begun by ----------at the University of California

• Richard Lenski

• Sewall Wright

• Ronald Fisher

• William Ratcliff

What were the main trends observed in the levels of DNA supercoiling relative to the ancestor?

• Increase

• Decrease

• Stable

What were the three populations that showed continued increases in supercoiling over time?

• Population A

• Population B

• Population C

What was the ancestral clone carrying during the experiment?

• Reporter plasmid pUC18

• Reporter plasmid pUC19

• Reporter plasmid pUC20

What does the absence of a bar for 6 at 10,000 generations indicate?

• Indicates a mutation

• Indicates stability

• Indicates a loss of function

What mutation occurs in the fis gene?

• Deletion

• Insertion

• Substitution

What is the effect of the evolved fis mutation on the Fis protein level?

• Increased

• Decreased

• No change

What do the changes in DNA topology suggest in the focal population?

• Increased mutation rate

• Stabilization of DNA

• Enhanced replication

What does the absence of a bar for 6 at 10,000 generations suggest?

• Indicates a mutation

• Indicates stability

• Indicates a gain of function

What implications do the changes in DNA topology have in the focal population?

• Increased mutation rate

• Stabilization of DNA

• Enhanced replication