Immunodiagnostics Quiz

Which immunodiagnostic method is based on having a pure antigen or antibody whose quantity can be measured by an indicator molecule?

• ELISA

• Microarrays

• Immunoblotting

• Flow cytometry

What is the main purpose of the sandwich assay in ELISA?

• To identify surface markers

• To detect antibodies

• To measure cytokines

• To determine antigen concentration

Which technique allows different cell populations to be identified simultaneously based on the proteins they express?

• Immunoblotting

• Flow cytometry

• Microarrays

• Real time PCR

What is the main function of the secondary response in the antigen-antibody reaction?

• To produce more antibodies than the primary response

• To activate naive B cells

• To trigger isotype switching

• To generate memory cells

Which immunodiagnostic method involves the detection of particles from different materials via electrophoresis followed by transfer to a membrane?

• Real time PCR

• Immunoblotting

• Flow cytometry

• Microarrays

What is the purpose of using multiple simultaneous fluorochromes in flow cytometry?

• To classify leukemia

• To detect surface parameters

• To identify nuclear parameters

• To analyze gene expression

In ELISA, what is the method referred to when the label is attached directly to the specific, primary antibody?

• Secondary

• Tertiary

• Indirect

• Direct

Which immunodiagnostic method is used to measure hundreds of proteins simultaneously using small volumes of clinical or biological samples?

• Real time PCR

• Immunoblotting

• Microarrays

• Flow cytometry

What is the main role of flow cytometry in acute leukemia diagnosis?

• To determine lineage

• To measure oxidative activity

• To detect surface markers

• To identify transcription factors

Which immunodiagnostic method involves the use of a DNA probe to measure gene expression?

• Real time PCR

• Immunoblotting

• Flow cytometry

• Microarrays