MSC Sem 3 Unit 3 +4

Which of the following accurately describes stable transfection?

• Temporary introduction of foreign DNA into cells with short-term gene expression.

• No integration of foreign DNA into the host genome, suitable for quick, high-level protein production.

• Permanent integration of foreign DNA into the host genome, with heritable and long-lasting genetic modification.

• Short-term gene expression lasting a few days, without the use of selection markers.

Which of the following statements correctly contrasts stable transfection and transient transfection?

• Stable transfection involves short-term gene expression without integration into the host genome, while transient transfection involves long-lasting genetic modification and permanent integration into the genome.

• Stable transfection is used for quick, high-level protein production and short-term experiments, while transient transfection is ideal for long-term studies and consistent protein expression.

• Stable transfection is characterized by no integration into the host genome and short-term gene expression, while transient transfection provides consistent protein expression through permanent genetic modification.

• Stable transfection involves permanent integration of foreign DNA into the host genome, allowing for heritable genetic modification, whereas transient transfection is characterized by temporary DNA introduction with short-term gene expression and no genome integration.

Which of the following components in HAT medium inhibits the de novo synthesis of purines and pyrimidines?

• Hypoxanthine

• Aminopterin

• Thymidine

• Folic acid

In Western blotting, what is used to detect the presence of a specific protein?

• Primary antibody and secondary antibody conjugated to an enzyme

• Restriction enzyme

• DNA probe

• RNA probe

What is the common feature of Southern, Northern, and Western blotting techniques?

• All detect RNA sequences

• All involve gel electrophoresis followed by transfer to a membrane

• All use nucleic acid probes for detection

• All measure protein-protein interactions

In Southern blotting, what is the role of the labeled DNA probe?

• To transfer proteins from the gel to the membrane

• To block non-specific binding sites on the membrane

• To hybridize with and detect the target DNA sequence

• To separate RNA fragments by size

In Western blotting, why is a blocking step performed after transferring proteins to the membrane?

• To enhance the chemiluminescence signal

• To prevent non-specific binding of antibodies

• To separate proteins by size

• To transfer proteins from the gel to the membrane

What is the purpose of the colony lift technique?

• To measure protein expression levels

• To screen bacterial colonies for specific DNA sequences

• To determine RNA stability

• To analyze protein-DNA interactions

In the colony lift technique, what is the role of the membrane after transferring bacterial colonies onto it?

• To separate DNA fragments by size

• To allow hybridization of the labeled probe with the target DNA

• To enhance the growth of bacterial colonies

• To extract RNA from the colonies

In the immunoprecipitation (IP) technique, which step follows the incubation of the lysate with a specific antibody against the target protein?

• Elute the target protein from the beads

• Add protein A/G beads to capture antibody-protein complexes

• Lyse cells to release proteins

• Wash beads to remove non-specific proteins