11 ATAR DNA extraction-validation revision

What is used to break apart the cells from each other when extracting dna from fruit?

• Lemon juice

• Vinegar

• Salt water

• Sugar water

What is added to the fruit soup to rupture the cell membranes and release the dna?

• Vinegar

• Bleach

• Ammonia

• Detergent

What is used to make the dna insoluble during the extraction process?

• Hot water

• Cold water

• Methanol

• Ethanol

What is the purpose of adding salt to the fruit soup during dna extraction?

• To remove the dna

• To make the dna more soluble

• To clump the dna together

• To break down the dna

What is the role of protease enzyme in the dna extraction process?

• To remove the dna

• To increase the dna length

• To break down the proteins around the dna

• To make the dna more soluble

What is the purpose of adding ice cold ethanol to the mixture during dna extraction?

• To break down the dna

• To slow down the action of enzymes

• To speed up the action of enzymes

• To remove the dna

Why do you not crush the strawberries too much?

• To maintain the natural colour of the strawberries

• It will cause the DNA to degrade

• To avoid releasing excess sugar

• To prevent contamination if you break the bad

What does crushing the strawberries do?

Answer explanation

It releases the nuclei where the DNA is stored.

What product in the process, breaks down the fatty membranes of the cell?

Answer explanation

The soap, found in the detergent

When the nuclear membrane is broken down, what is released into the solution?

Answer explanation

DNA