gene tech 1-5

What is the function of the enzyme DNA polymerase?

• to synthesise a polypeptide using mRNA as a template

• to synthesise a strand of DNA using a polypeptide as a template

• to synthesise a strand of DNA using DNA as a template

• to synthesise a strand of mRNA using DNA as a template

The following events occur in the replication of DNA. In which order do these events take place (first to last)?

• 1,5,3,2,4

• 1,3,5,2,4

• 3,1,5,4,2

• 5,1,3,4,2

Plasmid was extracted from a bacterial culture. 25 μL of the extract was diluted with 75 μL distilled water. The diagram below shows the absorbance of the diluted plasmid solution at different wavelengths. Which statement is correct?

• The DNA solution is heavily contaminated with RNA.

• Only DNA absorbs light at 260 nm.

• The DNA solution is heavily contaminated with protein.

• The DNA concentration in the undiluted extract is 22.5 mg/mL.

The following shows an outline of the transformation procedure. Which of the following correctly shows the purpose in performing each transformation step?

• Step 1: to break down bacterial cell wall

• Step 3: To allow the plasmids to come into close contact with the competent cells

• Step 4: to allow recombinant plasmid DNA to bind to bacterial cell surface

• Step 5: to allow cells to multiply and grow

Fig. 1.1 and Fig. 1.2 shows the restriction map for pK plasmid and the gene of interest Gene M respectively. Based on Fig. 1.1 and Fig. 1.2, determine the best pair of restriction enzymes to use for restriction digest.

• EcoRI

• BamHI

• XbaI

• KpnI

You need to ligate Gene M to pK plasmid. The digested plasmid concentration is 465 μg/ml and digested Gene M is 533 μg/ml. The table below states the quantity of pK plasmid and Gene M required in a ligation reaction. Determine the value of X, Y and Z.

note: gene M is 850bp