Understanding Gene Expression and Molecular Techniques

What is the primary purpose of studying gene expression in biology?

• To understand the chemical properties of cells

• To analyze the physical structure of cells

• To understand the expression of genomes in organisms

• To study the non-living components of cells

What is the role of reverse transcriptase in RT-PCR?

• It synthesizes mRNA from DNA

• It creates a complementary DNA strand from mRNA

• It enhances the reaction of polymerase

• It breaks down the DNA into smaller fragments

What is cDNA and how is it created?

• cDNA is a type of RNA that acts as an enzyme

• cDNA is double-stranded DNA synthesized from an mRNA template

• cDNA is single-stranded RNA created from mRNA

• cDNA is double-stranded DNA synthesized from a protein template

Why is DNA polymerase important in the creation of cDNA?

• It synthesizes the complementary DNA strand

• It breaks down the mRNA template

• It initiates the transcription process

• It labels the cDNA with fluorescent tags

How can RT-PCR be used to track gene expression during embryonic development?

• By measuring the physical changes in embryo cells

• By creating proteins specific to each stage of development

• By observing the color changes in embryo cells

• By isolating mRNAs at different stages and synthesizing cDNA

What does gel electrophoresis in RT-PCR indicate about gene expression?

• The presence and amount of specific cDNA

• The quantity of proteins in the sample

• The types of cells in the sample

• The temperature at which genes are expressed

What is the purpose of using specific primers in RT-PCR?

• To ensure only specific cDNA is amplified

• To break down unwanted DNA fragments

• To speed up the reaction process

• To label the cDNA fragments

Why is it important to understand gene expression in different tissues?

• To determine the color of the tissues

• To understand tissue-specific functions

• To change the genetic structure of tissues

• To observe the size of tissues

How does the DNA microarray assay help in understanding genome-wide expression?

• By hybridizing labeled cDNAs with complementary DNA sequences

• By altering the DNA structure in cells

• By cooling the cells to observe changes

• By attaching proteins to a solid surface

What does the presence of a specific color in a DNA microarray indicate?

• The age of the cells being tested

• The size of the DNA fragments

• The presence of a specific gene expression

• The temperature of the sample