CRISPR -Gene editing

What does CRISPR stand for?

• Combined RNA Interfered Splicing Palindromic Reactions

• Combined Regularly Interspaced Short Palindromic Repeats

• Clustered Randomly Interspaced Short Palindromic Reactions

• Clustered Regularly Interspaced Short Palindromic Repeats

Tags

NGSS.HS-LS3-2

What is the main function of the Cas-9 enzyme in the CRISPR-Cas9 system? (IMPORTANT)

• To bind to the RNA and form a complex

• To act as a pair of 'molecular scissors' to cut DNA

• To repair the DNA after it has been cut

• To identify the specific DNA sequence to be edited

What is the role of guide RNA (gRNA) in the CRISPR-Cas9 system? (IMPORTANT)

• To act as a 'molecular glue' for DNA strands

• To locate and attach to a specific sequence in the DNA

• To duplicate the DNA sequence

• To repair the DNA after cutting

What makes CRISPR-Cas9 a revolutionary gene editing technique?

• Its role in enhancing the flavor of water

• Its ability to change the color of plants

• Its speed, cost-effectiveness, and precision

• Its use in creating new computer programs

What is the primary purpose of gene editing ?

• To create decorative plants

• To increase the size of fruits and vegetables

• To study the history of DNA

• To treat diseases and improve agriculture

Which organism's DNA does CRISPR naturally occur in?

• Mammals

• Plants

• Archaea and bacteria

• Viruses

What is a unique feature of the CRISPR technique besides editing DNA sequences?

• Creating new elements

• Turning genes on or off without altering their sequence

• Teleporting genes from one organism to another

• Making organisms invisible

What is the function of the Cas9 protein (enzyme)? (IMPORTANT)

• To help unzip and unwind the DNA as well as keeping the strands apart so that the gene of interest can be located

• To locate a gene of interest with a complementary binding site,

• To act as a pair of 'molecular scissors' that can cut the two strands of DNA at a specific location in the genome so that bits of DNA can then be added or removed.

• As part of the CRISPR system, it guides important tools to a specific location on the genome.

How does Cas9 identify the target DNA sequence?

Answer choices

• Through direct protein-DNA interaction

• Using a guide RNA

• By recognizing specific protein markers

• Using a DNA repair mechanism

What is the natural function of Cas9 in bacteria?

• Defense against DNA viruses and plasmids

• Facilitating bacterial conjugation

• Promoting bacterial mobility

• Enhancing nutrient absorption