Bacteria Lab Prep

What name is given to this device?

Tags

NGSS.HS-PS3-4

What is the Autoclave used for?

• To dissolve the agar for pouring into petri plates

• To sterlize the agar for pouring into petri plates

• To mix the agar for pouring into petri plates

• To cook soup

In which order should the events take place to ensure a sterile environment for growing bacteria?

• Put on Gloves

• Wipe Down Table with 10% Bleach solution

• Wash Hands using Soap for a minimum of 30 seconds

• Collect a Petri Plate

• Label the Bottom of the Petri plate

When innoculating a petri plate (placing bacteia on the plate), which of the following images shows the proper method.

• 
• 
• 
• 

In this image of a person innoculating a petri plate (placing bacteia on the plate), label the correct procedures.

a

b

c

Which sequence should you follow in lab to innoculate your own petri plate?

• Retrieve a Closed Petri Plate

• Turn over the Petri Plate and Label and Divide the Bottom with a Wax Pencil

• Wet a Sterile Q-tip with water

• Sterilize Surface, Sterilize Hands, and Put on Gloves

• Swab The Surface You wish to Test

After swabbing the surface you wish to test, what are the next steps in order?

• Close the Cover

• Gently swab the innoculated QTip across the surface of the agar

• Using a Forceps, dip a Paper Disk into an antibacterial solution of your choice.

• Open the Petri Dish Cover 1/3 of the way

• Open the Petri Dish Cover 1/3 of the way and place the disk on the agar on one side of the dividing line

Which of the following procedures, should you follow before putting your plate in the incubator?

• Tape all four corners of the Petri Dish

• Turn the Petri Dish upside Down

• Do not Tape the Petri Dish

• Leave the Petri Dish with the Cover Facing up.