Bacteria Lab Prep

What name is given to this device?

Tags

NGSS.HS-PS3-4

What is the Autoclave used for?

• To dissolve the agar for pouring into petri plates

• To sterlize the agar for pouring into petri plates

• To mix the agar for pouring into petri plates

• To cook soup

In which order should the events take place to ensure a sterile environment for growing bacteria?

• Wipe Down Table with 10% Bleach solution

• Collect a Petri Plate

• Wash Hands using Soap for a minimum of 30 seconds

• Label the Bottom of the Petri plate

• Put on Gloves

When innoculating a petri plate (placing bacteia on the plate), which of the following images shows the proper method.

• 
• 
• 
• 

In this image of a person innoculating a petri plate (placing bacteia on the plate), label the correct procedures.

a

b

c

Which sequence should you follow in lab to innoculate your own petri plate?

• Wet a Sterile Q-tip with water

• Sterilize Surface, Sterilize Hands, and Put on Gloves

• Swab The Surface You wish to Test

• Retrieve a Closed Petri Plate

• Turn over the Petri Plate and Label and Divide the Bottom with a Wax Pencil

After swabbing the surface you wish to test, what are the next steps in order?

• Open the Petri Dish Cover 1/3 of the way

• Open the Petri Dish Cover 1/3 of the way and place the disk on the agar on one side of the dividing line

• Close the Cover

• Using a Forceps, dip a Paper Disk into an antibacterial solution of your choice.

• Gently swab the innoculated QTip across the surface of the agar

Which of the following procedures, should you follow before putting your plate in the incubator?

• Tape all four corners of the Petri Dish

• Turn the Petri Dish upside Down

• Do not Tape the Petri Dish

• Leave the Petri Dish with the Cover Facing up.