Unit 4: DNA Replication. 3

What is the role of helicase in DNA replication?

• Synthesizes new DNA strands

• Unwinds the double helix and separates the strands

• Cuts the phosphate backbone

• Adds nucleotides to the growing DNA strand

Which enzyme is responsible for adding new nucleotides to the 3' end of the DNA?

• Primase

• DNA polymerase

• Helicase

• DNA ligase

What is the direction of DNA replication?

• 3' to 5'

• Both 5' to 3' and 3' to 5'

• 5' to 3'

• Random direction

What are Okazaki fragments?

• RNA primers

• Short DNA fragments on the leading strand

• Short DNA fragments on the lagging strand

• Enzymes involved in DNA replication

What is the first step of DNA replication?

• Separation

• Elongation

• Initiation

• Termination

Which enzyme relieves stress on the DNA molecule during replication?

• Single-Strand Binding Proteins

• Helicase

• DNA polymerase

• Topoisomerase

What is the role of primase in DNA replication?

• Adds nucleotides to the 3' end

• Joins Okazaki fragments

• Unwinds the DNA helix

• Synthesizes RNA primers

What is semiconservative replication?

• Both strands of DNA are conserved

• Each new DNA molecule contains one old and one new strand

• Only one strand of DNA is replicated

• DNA is replicated in a conservative manner

Where does initiation of DNA replication occur?

• At the replication fork

• At origins of replication

• At the 3' end

• At the 5' end

What is the role of DNA ligase in DNA replication?

• Unwinding the DNA helix

• Synthesizing RNA primers

• Adding nucleotides to the 3' end

• Joining Okazaki fragments