Teste de Suscetibilidade Antimicrobiana

What does MIC stand for in the context of antimicrobial susceptibility testing?

• Maximum Inhibitory Concentration

• Minimum Inhibitory Capacity

• Minimum Inhibitory Concentration

• Mean Inhibition Count

Explain how the MIC determination is carried out in the laboratory.

• Use a higher concentration of the antimicrobial agent to determine the MIC

• Incubate the mixture at room temperature for 24 hours

• Mix the antimicrobial agent with the target microorganism without dilution

• Prepare a series of dilutions of the antimicrobial agent in a liquid medium, add a standardized inoculum of the target microorganism to each dilution, incubate, and determine the MIC as the lowest concentration that inhibits visible growth.

What is the E-test method used for in antimicrobial susceptibility testing?

• Determining the minimum inhibitory concentration (MIC) of an antimicrobial agent against a specific microorganism.

• Identifying the species of a microorganism

• Testing the electrical conductivity of a substance

• Measuring the pH of a solution

Describe the procedure involved in performing the E-test method.

• Performing the E-test method includes preparing the agar plate with the target bacteria, placing the E-test strip on the plate, incubating the plate, and then reading the MIC value where the bacterial growth intersects the strip.

• Using a different type of agar plate for the E-test method

• Mixing the E-test strip with water before placing it on the agar plate

• Reading the MIC value before incubating the plate

How does the agar dilution method work in determining antimicrobial susceptibility?

• The agar dilution method determines antimicrobial susceptibility by assessing the color change of the agar

• The agar dilution method determines antimicrobial susceptibility by identifying the minimum inhibitory concentration (MIC) of the antimicrobial agent.

• The agar dilution method works by measuring the pH of the agar medium

• The agar dilution method involves counting the number of bacterial colonies on the agar plate

What are the advantages of using the agar dilution method?

• Limited availability of agar

• Time-consuming process

• Subject to human error

• Accurate and reliable determination of minimum inhibitory concentration (MIC) values.

What factors can influence the accuracy of MIC determination results?

• Color of the testing plate

• Brand of the laboratory equipment used

• Time of day the test is conducted

• Choice of antimicrobial agent, testing method, inoculum size, incubation conditions, interpretation criteria

Compare and contrast the E-test method with the agar dilution method.

• The E-test method involves using a series of agar plates with different antibiotic concentrations, while the agar dilution method uses a predefined gradient of antibiotic concentrations on a strip.

• Both the E-test method and the agar dilution method require the use of liquid media for antibiotic susceptibility testing.

• The E-test method is more time-consuming than the agar dilution method due to the need for manual interpretation of results.

• The E-test method uses a predefined gradient of antibiotic concentrations on a strip, while the agar dilution method requires preparing a series of agar plates with different antibiotic concentrations.

Discuss the importance of interpreting MIC values correctly in clinical settings.

• MIC values are only used for research purposes

• Incorrect MIC interpretation leads to better patient outcomes

• Interpreting MIC values is irrelevant in clinical settings

• Interpreting MIC values correctly is essential for determining effective antibiotic treatment and avoiding treatment failure, resistance, and side effects.

How can antimicrobial susceptibility testing help in guiding treatment decisions for bacterial infections?

• Antimicrobial susceptibility testing provides information on which antibiotics are effective against the specific bacteria causing the infection, guiding healthcare providers in selecting the most appropriate treatment.

• Antimicrobial susceptibility testing analyzes the patient's genetic predisposition to infections

• Antimicrobial susceptibility testing predicts the weather conditions for the patient's recovery

• Antimicrobial susceptibility testing determines the patient's blood type