Emerging Lab Technologies

I have a tissue sample. I want to know whether my protein of interest is located in the membrane or in the nucleus. Which technique should I use?

• IHC

• ELISA

• Western blot

Which -ome does RNA-seq tell you about?

• genome

• transcriptome

• proteome

Why do we use multiple types of antibodies in immuno assays?

• To amplify the signal

• To identify multiple antigens

• To bind multiple antibodies to the same antigen

What causes DNA to migrate across the gel?

• Gravity

• Osmosis

• Electricity

• Diffusion

Tags

NGSS.HS-PS2-5

NGSS.HS-PS3-5

What does PCR stand for?

• Polypeptide Chemical Response

• Polymerase Chain Reaction

• Protein Complex Restriction

• Pipette, Cry, Repeat

Which molecule cuts DNA?

• CRISPR

• Cas9

• guide RNA

• Taq polymerase

Which technique(s) can be used to characterize cancer cells?

• RNA-seq

• CRISPR-Cas9

• IHC

Put the steps of PCR in order: DNA is ___, then primers are ___ and ____. 

• denatured, annealed, extended

• annealed, denatured, extended

• extended, denatured, annealed

Which technique(s) involves separating biomolecules based on their size?

• Western blot

• Gel electrophoresis

• NMR

Complete the steps of NMR: ___ aligns the spins, ____ causes resonance.

• stable magnetic field, oscillating magnetic field

• nuclear magnetic field, molecular magnetic field

• nuclear magnetic field, oscillating magnetic field