Animal Cell Culture

The stage of cell culture after the isolation of the cells but before the first subculture is known as

• Primary culture

• Passaging

• Secondary culture

• All

What is the purpose of media preparation in animal cell culture?

• To keep the cells warm during the experiment

• To test the pH levels of the cells

• To provide the necessary nutrients, growth factors, and environment for the cells to grow and thrive.

• To provide entertainment for the researchers

Why is it important to maintain sterile techniques in cell culture?

• To promote genetic mutations

• To increase cell growth rate

• To prevent contamination and ensure accurate results.

• To save time and effort

What are the key components of a cell culture media?

• Enzymes, hormones, antibodies, and cytokines

• Lipids, nucleic acids, carbohydrates, and water

• Minerals, fatty acids, nucleotides, and coenzymes

• Amino acids, vitamins, inorganic salts, glucose, growth factors, and serum

How can contamination be prevented during cell culture experiments?

• Handle cell cultures roughly

• Maintain sterile conditions, use aseptic techniques, clean work surfaces and equipment, handle cell cultures properly.

• Ignore aseptic techniques

• Use dirty work surfaces and equipment

Which of the following is Not the essential equipment for cell culture?

• Cell culture hood

• Centrifuge

• Spectrophotometer

• Incubator

What is the purpose of adding trypsin to cell culture in the experiment?

• Division of cells

• Culture of cells

• Staining of cells

• Deaggregation of cells

In the secondary culture, cells are obtained from

• primary culture

• the organism

• organ culture

• phenotypic culture

Which of the following proteins acts as in animal cell culture

• Fibronectin

• Albumin

• Chondronectin

• all of the above

Fibronectin can be added to serum free media

• for amino acid supplementation

• for carrying iron

• for cell matrix interaction

• for carrying lipoproteins