What are drawn through pores in the gel during agarose gel electrophoresis?

Agarose Gel Electrophoresis Quiz

Quiz
•
Biology
•
12th Grade
•
Medium
Andrea Clarke
Used 1+ times
FREE Resource
33 questions
Show all answers
1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Proteins and lipids
DNA and RNA
Carbohydrates and vitamins
Amino acids and enzymes
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the basis for separation in agarose gel electrophoresis?
The color of the species
The electrical conductivity of the species
The size and charge of the species
The solubility of the species in the gel
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of applying current to a medium in agarose gel electrophoresis?
To heat the medium
To create an electrical field that causes species to migrate
To solidify the agarose gel
To visualize the DNA and RNA without a UV light
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is electrophoresis?
A technique that uses electrical current to separate DNA, RNA, or proteins based on their size and charge.
A method for heating substances to determine their melting points.
A process for combining different DNA, RNA, or proteins into a single mixture.
A technique for visualizing cellular structures under a microscope.
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of agarose gel electrophoresis?
To amplify DNA fragments
To separate nucleic acid fragments based on their size
To visualize proteins using UV light
To create secondary structures of RNA
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Why might bands observed on native agarose gels not always represent the true sizes of RNA?
Because RNA does not migrate through agarose gels
Because RNA fragments are positively charged
Because RNA tends to form secondary structures that affect migration
Because the electrical current is not applied properly
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Why are proteins often separated on polyacrylamide gels instead of agarose gels?
Because polyacrylamide gels have larger pores
Because proteins do not migrate through agarose gels
Because polyacrylamide gels offer greater resolution for small protein molecules
Because proteins are not charged molecules
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