Agarose Gel Electrophoresis Quiz

Agarose Gel Electrophoresis Quiz

12th Grade

33 Qs

quiz-placeholder

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Agarose Gel Electrophoresis Quiz

Agarose Gel Electrophoresis Quiz

Assessment

Quiz

Biology

12th Grade

Medium

Created by

Andrea Clarke

Used 1+ times

FREE Resource

33 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What are drawn through pores in the gel during agarose gel electrophoresis?

Proteins and lipids

DNA and RNA

Carbohydrates and vitamins

Amino acids and enzymes

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the basis for separation in agarose gel electrophoresis?

The color of the species

The electrical conductivity of the species

The size and charge of the species

The solubility of the species in the gel

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of applying current to a medium in agarose gel electrophoresis?

To heat the medium

To create an electrical field that causes species to migrate

To solidify the agarose gel

To visualize the DNA and RNA without a UV light

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is electrophoresis?

A technique that uses electrical current to separate DNA, RNA, or proteins based on their size and charge.

A method for heating substances to determine their melting points.

A process for combining different DNA, RNA, or proteins into a single mixture.

A technique for visualizing cellular structures under a microscope.

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of agarose gel electrophoresis?

To amplify DNA fragments

To separate nucleic acid fragments based on their size

To visualize proteins using UV light

To create secondary structures of RNA

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Why might bands observed on native agarose gels not always represent the true sizes of RNA?

Because RNA does not migrate through agarose gels

Because RNA fragments are positively charged

Because RNA tends to form secondary structures that affect migration

Because the electrical current is not applied properly

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Why are proteins often separated on polyacrylamide gels instead of agarose gels?

Because polyacrylamide gels have larger pores

Because proteins do not migrate through agarose gels

Because polyacrylamide gels offer greater resolution for small protein molecules

Because proteins are not charged molecules

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