Enzymes That Manipulate DNA & CRISPR

The DNA base sequences recognized by Endonuclease are called

• Restriction Site

• Recognition Site

• Cutting Site

• Introns & Exons

Restriction Enzymes are isolated from :

• Viruses

• Bacteria

• Protozoans

• Fungi

The enzyme used to join the DNA fragments

• DNA Ligase

• RNA Polymerase

• DNA Polymerase

• Restriction Endonuclease

Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its uses?

• restriction endonuclease - production of DNA fragments for gene cloning.

• 
  reverse transcriptase - production of cDNA from mRNA.

• Taq polymerase - copies DNA sequences in the polymerase chain reaction.

• DNA ligase - enzyme that cuts DNA, creating sticky ends.

Which is true about plasmids?

• Plasmids can be cut at specific sequences called restriction sites.

• 
  Plasmids contain a promoter sequence that defines where transcription begins.

• 
  Plasmids can contain an antibiotic resistance gene.

• All of the statements are true about plasmids.

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• Insertion

• Amplification

• Transformation

• Screening

EcoRI and Sma1 are example of which type of enzymes?

• Ligases

• Polymerases

• Endonucleases

• Englishphrases

In the diagram, the yellow molecule represents an enzyme. The enzyme best represented would be:

• DNA Polymerase

• Endonuclease

• DNA Ligase

• RNA Polymerase

_________ Polymerase is used in the replication of DNA from a pre-existing DNA strand

A _________ is a short single-strand of complementary nucleic acid