Biotechnology Notes REVIEW

Enabled scientists to read the genetic code for the first time. Ideal for longer DNA sequences up to 900 base pairs.

• Human Genome Project

• Sanger Sequencing

• Restriction Enzymes

• Gene Therapy

an enzyme that is used to connect 2 different strands of DNA together assisting in inserting genes and other pieces of DNA into plasmids.

• Gel Electrophoresis

• DNA Ligase

• Sanger Sequencing

• GMO

1. The use of living organisms, cells, and molecules to create products and solve problems for the benefit of humankind.

• Synthetic Biology

• Restriction Enzymes

• Biotechnology

• PCR

A treatment used to replace a harmful gene with a functioning one in an effort to treat or stop disease, uses bacterial plasmids.

• PCR

• DNA LIGASE

• CRISPR

• Gene Therapy

A protein that is capable of recognizing a specific pattern of nucleotides and used to break a strand of DNA at a specific location.

• Restriction Enzyme

• Gene Therapy

• DNA Ligase

• Sanger Sequencing

Allows a single or a few copies of DNA to be replicated into millions or billions of copies. And by amplifying that DNA, it allows us to study that DNA molecule in detail in the laboratory.

• PCR

• Restriction Enzyme

• Sanger Sequencing

• Gel Electrophoresis

An enzyme that is used to connect 2 different strands of DNA together assisting in inserting genes and other pieces of DNA into plasmids

• RNA polymerase
• ATP synthase
• Glycogen phosphorylase
• DNA ligase

Identified all the genes and their functions that make up the human genome (30,000).

• The Human Gene Encyclopedia
• The Human Genome Encyclopedia
• The Human Genetic Code Project
• The Human Genome Project

Which Biotechnology method would a scientist use to treat Sickle Cell Disease?

• Gene therapy
• Physical therapy
• Radiation therapy
• Chemotherapy

Uses an enzyme (Cas9) to altering 

DNA sequences and modifying gene function

• CRISPR-Cas9
• Cell division
• Protein synthesis
• RNA interference