C5-3 Interpreting chromatograms

Many chromatograms do not contain coloured substances. Which of the following would NOT help you see the spots?

• UV light

• Ninhydrin

• Titrant

• Iodine (in a tank)

Which sum is used to calculate the Rf value of the red substance in the chromatogram?

• 3+5

• 5/3

• 5-3

• 3/5

What is the Rf value of spot B?

• 3/7

• 4/7

• 7/3

• 7/4

Calculate the Rf value for spot A.

Calculate the Rf value for spot B.

Calculate the Rf value for spot C.

Calculate the Rf value for the red spot.

Calculate the Rf value for the blue spot.

What does the unknown material contain?

• A,B & C

• A, C & another substance

• unknown substances

• B & C only

The green spot (B) had an Rf value of 0.33. If the distance travelled by the spot was 3 cm, what was the distance to the solvent front?

• 0.11 cm

• 2.67 cm

• 9 cm

• 10cm