TOOLS IN GENETIC ENGINEERING

Why DNA technology important?

• Allows the manipulation of DNA to create new genetically modified organisms

• Create new type of viruses and medicines to control DNA

• Maintain DNA in an organisms and make structure stable

• To isolate DNA in a laboratory

Which of the following pair is correctly match?

• I - b; II - a; III - c; IV - d; V - e

• I - a; II - e; III - d; IV - b; V - c

• I - b; II - a; III - e; IV - d; V - c

• I - e; II - b; III - c; IV - d; V - a

What is the purpose of plasmid DNA isolation in molecular cloning?

• To obtain pure plasmid DNA for use in molecular cloning techniques

• To use as a food additive

• To study the structure of plasmid DNA

• To create a new species of bacteria

Which of the following describes the role of restriction enzymes in the process of molecular cloning?

• Restriction enzymes are used to amplify DNA in PCR

• Restriction enzymes cut DNA at specific sequences, allowing for the insertion of foreign DNA into a plasmid or vector.

• 
  Restriction enzymes help in transcribing DNA into RNA

• 
  Restriction enzymes are involved in protein synthesis

What is ligation and how is it used in molecular cloning?

• Ligation is the process of editing DNA fragments using DNA ligase. In molecular cloning, ligation is used to modify the genetic code of a DNA molecule.

• Ligation is the process of breaking DNA fragments apart using DNA ligase. In molecular cloning, ligation is used to separate DNA strands for analysis.

• Ligation is the process of joining DNA fragments together using DNA ligase. In molecular cloning, ligation is used to insert a DNA fragment into a vector to create a recombinant DNA molecule.

• Ligation is the process of duplicating DNA fragments using DNA ligase. In molecular cloning, ligation is used to create multiple copies of a DNA sequence.

Which of the following describe the process of transforming bacterial cells in molecular cloning.

• 
  Introduce foreign proteins into the bacterial cells using techniques such as watering them with sugar water

• Introduce foreign DNA into the bacterial cells using techniques such as singing to them

• Introduce foreign DNA into the bacterial cells using techniques such as heat shock, electroporation, or chemical transformation.

• Introduce foreign RNA into the bacterial cells using techniques such as baking them in the oven

What methods can be used to verify cloned DNA in molecular cloning?

• Western blotting, Southern blotting, and Northern blotting

  Gel electrophoresis, Centrifugation, and

• Gel electrophoresis, Centrifugation, and Spectrophotometry

• ELISA, Flow cytometry, and Immunohistochemistry

• restriction enzyme digestion, DNA sequencing, and PCR

What are the key steps involved in plasmid DNA isolation?

• Cell lysis, removal of proteins and RNA, and precipitation of DNA

• DNA synthesis, cell lysis, and removal of RNA

• Protein precipitation, DNA purification, and cell lysis

• Cell lysis, purification of proteins, and degradation of DNA

How do restriction enzymes recognize specific DNA sequences for digestion?

• By their ability to change the DNA sequence

• By their interaction with lipids in the cell membrane

• By their unique protein structure and binding sites

• By their preference for RNA sequences

What is the significance of using antibiotic resistance markers in the selection of transformed cells?

• They increase the mutation rate in transformed cells

• They reduce the efficiency of transformation

• They prevent the growth of transformed cells

• They allow for the identification and isolation of transformed cells from non-transformed cells.