DNA Technology

What does the term PCr stand for?

• Polymerase Chain Reaction

• Preliminary Chain Reaction

• Polymerase Coiled Reaction

• Protein Chain Reaction

Explain the process of gel electrophoresis.

• Gel electrophoresis separates RNA fragments based on their size and charge by running them through a liquid matrix using an electric field.

• Gel electrophoresis separates DNA fragments based on their color and texture by running them through a gel matrix using an electric field.

• Gel electrophoresis separates DNA fragments based on their size and charge by running them through a gel matrix using an electric field.

• Gel electrophoresis separates proteins based on their shape and weight by running them through a gel matrix using a magnetic field.

How is recombinant DNA technology used in medicine?

• To improve crop yield through genetic modification

• To produce therapeutic proteins through genetic engineering

• To create new species of animals

• To develop new types of antibiotics

What is the significance of DNA fingerprinting in forensic science?

• It helps in identifying individuals, solving crimes, and exonerating the innocent.

• It is not a reliable method for identifying individuals

• It is only used for entertainment purposes

• It has no significance in forensic science

What is gene cloning?

• Mixing different genes together

• Creating multiple copies of a gene or DNA sequence.

• Using a microscope to view genes

• Deleting a gene from the DNA sequence

What are the three main steps of PCR?

• Cutting, Pasting, Copying

• Denaturation, Annealing, Extension

• Heating, Cooling, Reading

• Mixing, Separation, Amplification

How does gel electrophoresis separate DNA fragments?

• By using a magnetic field to separate the DNA fragments

• By heating the gel to separate the DNA fragments

• By adding chemicals to the gel to separate the DNA fragments

• By applying an electric field to the gel, causing the DNA fragments to move towards the positive electrode at different rates based on their size and charge.

Explain the role of restriction enzymes in recombinant DNA technology.

• Restriction enzymes help in DNA replication

• Restriction enzymes are used to produce ATP in cells

• Restriction enzymes cut DNA at specific sequences, allowing for the insertion of foreign DNA into a plasmid or vector.

• Restriction enzymes are involved in protein synthesis

In gel electrophoresis, which direction do DNA fragments or proteins migrate in the gel?

• Toward the positive electrode

• Toward the negative electrode

• Toward the center of the gel

• They do not migrate; the gel remains stationary

Which of the following enzyme is used to cut DNA molecule during Gene Cloning?

• DNA polymerase

• Ligase

• Phosphates

• Restriction enzymes