Sanger Sequence

Which of the following is NOT found in a DNA nucleotide?

• Uracil

• Phosphate

• Base

• Deoxyribose sugar

What enzyme unzips the double strand of DNA?

• DNA polymerase

• helicase

• RNA polymerase

• Tryptophan

Which of the following will Thymine pair with?

• Adenine

• Uracil

• Cytosine

• Guanine

What part of sanger sequencing is fluorescently labeled?

• Nucleotides

• sugar

• DNA polymerase

• dideoxynucleotides

Because we need the DNA strand to stop growing, what do we need to add?

• a primer

• DNA polymerase

• Dideoxynucleotides

• template DNA

How do we break apart the double stranded DNA during the sanger sequence?

• helicase

• DNA polymerase

• heat

• Taq polymerase