Lecture 9

Antigen is a molecule from a pathogen or foreign organism

that ‘provoke’ a specific immune response.

• True

• False

Hapten is a region in an antigen that interacts with an antibody.

• True

• False

Antibody is protein that recognize and bind to a

particular antigen with very high specificity.

• True

• False

Antibody and antigen interact by lock and key manner.

• True

• False

Single diffusion (radial immunodiffusion) measures proteins called immunoglobulins in the blood.

• True

• False

Agglutination's principle:
Particulate antigen + antibody –> clumping

• True

• False

Enzyme-linked immunosorbent assay (ELISA) is a technique to isolate and

concentrate a particular protein from a sample containing many thousands of different proteins.

• True

• False

Immunoprecipitation (IP) used mainly in immunology to detect the

presence of an antibody or an antigen in a sample.

• True

• False

Antigens isolated by Immunoprecipitation (IP) are

analyzed by SDS-PAGE or Western blotting.

• True

• False

Types of ELISA:

1) Direct ELISA

2) Indirect ELISA

3) Competitive ELISA

4) Sandwich ELISA

• True

• False