Lecture 8

Flowcytometry: Technique to measure properties of cells as they flow in a fluid suspension across a laser

light path

• True

• False

Properties measured are: cell size, relative granularity or internal complexity, and relative fluorescence intensity

• True

• False

A flow cytometer is composed of fluidic (flow), optic, and electronic systems

• True

• False

Principle of hydrodynamic focusing:

All cells can pass through the laser beam at a given moment.

• True

• False

in SIDE SCATTER (SSC), the intensity of the signal

is proportional to cell size

• True

• False

For FORWARD SCATTER (FSC), the intensity of the signal

is proportional to the complexity/ granularity of the cell

• True

• False

The electronic system is used to identify different type of cells

• True

• False

Fluorophores used for the detection of target proteins

emit light after excitation by a laser of compatible wavelength

• True

• False

Sorters are used to analyze particular type of cells qualitatively and quantitatively.

• True

• False

Here is the clinical application in transfusion and transplant:

• HLA B27 assay

• Detection of MRD

• Cytokine response

• True

• False