Quiz Agrobacterium mechanism

Which one is WRONG about Agrobacterium tumefaciens?

• Gram-negative bacteria

• Gram-positive bacteria

• Soil borne bacteria

• Rod-shaped bacterium

Oncogenic genes are near to right T-DNA border

• Yes

• No

Determine the function of Vir region in Ti-plasmid

• Encode proteins involved in the production of octopines

• Genes responsible for transfer of T-DNA into plant genome

• Encode proteins involved in the production of the auxin

• Involve in determining tumour size in some species

Which one is NOT a common features of Ti-Plasmid

• Contain one vir region

• Contain one (or more) T-DNA regions

• Contain an origin of replication

• Contain genes for the anabolism of opines

Function of virA protein

• Transcriptional factor

• Components of membrane structure for T-DNA transfer

• Phenolic sensor

• Nick the T-DNA

Function as transcriptional factor to induces expression of all vir genes

• vir B1

• vir G

• vir D2

• vir E

This Vir protein produces single-stranded nicks in the DNA

• vir F

• vir B2

• vir D2

• vir D4

Vir E2 and Vir A also exported from the bacterial cell into plant cell

• Yes

• No

Function of Vir E2 molecules in plant cells

• protect the T-DNA from nucleases

• facilitate nuclear localization

• confer the correct formation to the T-DNA-VirD2 complex

• degrade the Vir proteins

Vir D2 contains nuclear localization signal and does notinteracts with a plant protein

• Yes

• No