
Metro Micro Final lab prac
Quiz
•
Biology
•
University
•
Practice Problem
•
Easy
Standards-aligned
Elizabeth Medina
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30 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
The following gene was used for sequencing because it is highly conserved therefore differences in sequence are considered significant
16s rRNA
5srRNA
Cell wall
LPS
Catalase
2.
MATCH QUESTION
1 min • 1 pt
Match the PCR steps
Reduce temperature to bind primers
Annealing
72C allows DNA polymerase to function
Denaturing step
Heat DNA at 95C to separate DNA strands
Extension
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which of the following is not used in traditional PCR
dNTPs
Primers
Heat-stable DNA polymerase
ddNTPs
Tags
NGSS.HS-PS1-2
4.
MULTIPLE SELECT QUESTION
45 sec • 1 pt
Which of the following are true of ddNTPs?
The 2' OH is replaced with H
The 3' OH is replaced with H
Fluorescent marker attached
Can continue the DNA chain
5.
MATCH QUESTION
1 min • 1 pt
Match the QC report terms with their definition.
Number of base pairs in the sequence
QV20+
Number of bases with QC of 20 or higher
Length
Probability that reads are correct
QC
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
In this chromatogram, which nucleotide has a low QC?
A
G
T
C
7.
MULTIPLE SELECT QUESTION
45 sec • 1 pt
Which test strips are valid?
A
B
C
D
E
Tags
NGSS.HS-PS1-2
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