Metro Micro Final lab prac

The following gene was used for sequencing because it is highly conserved therefore differences in sequence are considered significant

• 16s rRNA

• 5srRNA

• Cell wall

• LPS

• Catalase

Match the PCR steps

• Reduce temperature to bind primers

• Annealing

• 72C allows DNA polymerase to function

• Denaturing step

• Heat DNA at 95C to separate DNA strands

• Extension

Which of the following is not used in traditional PCR

• dNTPs

• Primers

• Heat-stable DNA polymerase

• ddNTPs

Tags

NGSS.HS-PS1-2

Which of the following are true of ddNTPs?

• The 2' OH is replaced with H

• The 3' OH is replaced with H

• Fluorescent marker attached

• Can continue the DNA chain

Match the QC report terms with their definition.

• Number of base pairs in the sequence

• QV20+

• Number of bases with QC of 20 or higher

• Length

• Probability that reads are correct

• QC

In this chromatogram, which nucleotide has a low QC?

• A

• G

• T

• C

Which test strips are valid?

• A

• B

• C

• D

• E

Tags

NGSS.HS-PS1-2

Which of the following is a positive result?

• A

• B

• C

• D

The lateral flow assay determines if an antigen is present.

• False

• True

Nosocomial infections are

• HAI

• infections acquired in a hospital setting

• Infections acquired outside of a hospital

• infections of the upper respiratory tract