CCLC QUIZ

In ion exchange chromatography, the stationary phase's functional groups primarily interact with:

• Neutral molecules

• Polar molecules

• Charged molecules

• Aromatic molecules

What defines the selectivity of an ion exchange resin in chromatography?

• Ionic strength of the eluent

• Nature of counter ions

• Particle size of the resin

• pH of the mobile phase

Ion exchange chromatography separates analytes based on differences in their:

• Charge distribution

• Molecular weight

• Solubility

• Boiling point

In ion exchange chromatography, what effect does an increase in ionic strength of the eluent have on the analyte's retention times?

• Decreases retention times

• Increases retention times

• No effect on retention times

• Unpredictable effect on retention times

Which ion exchange chromatography mode separates cations based on their affinity for the resin?

• Anion-exchange chromatography

• Cation-exchange chromatography

• Bipolar chromatography

• Hydrophobic interaction chromatography

Silica gel and alumina are commonly used stationary phases in which type of chromatography?

• Adsorption

• Partition

• Ion exchange

• Size exclusion

The interaction in RP HPLC is considered

• hydrophilic

• Hydrophobic

• ionic

• hydrogen bond

Which is NOT HPLC types using ion interaction mechanism

• cation exchange chromatography

• anion exchange chromatography

• ion exclusion chromatography

• HILIC

Which is correct about size exclusion chromatography?

• The stationary phase contain different pore sizes

• smaller molecules will be eluted earlier

• smaller molecules will be eluted later

• separation is based on different abilities of analytes to access mobile phase within the pores