MI4.1.2e Analysis of GFP / SDS-PAGE

The PA in PAGE stands for ________.

The GE in PAGE stands for ________.

PAGE can be used to assess the ________ of a protein. (Choose all that apply.)

• purity

• presence

• size

• shape

• amino acid composition

Place the following steps of PAGE in order.

• samples are loaded into the wells

• buffer is added to cover the gel

• electrical current is passed over the gel

• proteins migrate toward the positive electrode

• gel is placed into the electrophoresis chamber

In PAGE, ​ (a)   proteins will move farther on the gel and ​ (b)   proteins will not move as far. All proteins, however, will move toward the ​ (c)   electrode end of the gel.

• smaller
• larger
• positive
• negative

Proteins are (a)   made of linked ​ (b)   in a genetically-determined sequence. These subunits are linked by ​ (c)   bonds. A chain of linked subunits is called a ​ (d)   and when it is folded, it is called a ​ (e)  

• polymers
• amino acids
• peptide
• polypeptide
• protein.

Tags

NGSS.HS-LS1-1

NGSS.HS-LS1-6

Structural differences between proteins of a similar weight can cause them to migrate at different rates during PAGE.

• true

• false

To unfold a protein is to ________ it.

SDS is a ________

• detergent

• buffer

• protein

• gel

To break the strong disulfide bonds within a protein, ________ agents must be used, such as BME or DTT.