MI4.1.2b Genetic Engineering Using Recombinant DNA Tech

Small circular pieces of non-chromosomal DNA are known as ________

Bacterial plasmids contain essential genes.

• true

• false

B-lactamase is an enzyme that codes for bacterial resistance to ________ by degrading its molecules before they can break down the bacterial cell wall.

​ (a)   are bacterial ​ (b)   that carry genes into bacterial cells. They are created in a lab and contain genes from multiple different sources, thus qualifying them as ​ (c)   e

• Vectors
• plasmids
• recombinant DNA.
• chromosomal DNA
• chromosomes
• bacteria
• antibiotics

Recombinant DNA plasmids contain all of the following EXCEPT

• Origin of Replication

• Multiple Cloning Site

• Promoter

• Selectable Marker

• IPTG

The origin of replication is needed for a plasmid to be

• replicated

• conjugated

• transformed

• selected

The multiple cloning site contains ________ that allow easy insertion of genes and other DNA sequences into a plasmid.

• restriction sites

• restriction enzymes

• promoters

• chromosomes

• nucleotide pairs

Tags

NGSS.HS-LS3-2

The (a)   recruits ​ (b)   to the beginning of a ​ (c)   so that it can start the process of ​ (d)   ​

• promoter
• RNA polymerase
• gene
• transcription.
• translation
• DNA sequence
• DNA polymerase
• restriction enzymes