Chromatography Quiz

What are the two main types of chromatography?

• Solid chromatography and gel chromatography

• Column chromatography and affinity chromatography

• Gas chromatography and liquid chromatography

• Paper chromatography and thin-layer chromatography

Explain the principle of chromatography.

• Chromatography is a technique used to separate and analyze the components of a mixture based on their different affinities for a stationary phase and a mobile phase.

• Chromatography is a technique used to mix components together

• Chromatography has no use in scientific analysis

• Chromatography separates components based on their color

What is the difference between gas chromatography and liquid chromatography?

• Gas chromatography separates compounds based on their color, while liquid chromatography separates compounds based on their size.

• Gas chromatography separates compounds based on their solubility, while liquid chromatography separates compounds based on their boiling point.

• Gas chromatography separates compounds based on their reactivity, while liquid chromatography separates compounds based on their electrical charge.

• Gas chromatography separates compounds based on their volatility, while liquid chromatography separates compounds based on their affinity for the stationary phase.

How does gas chromatography work?

• Gas chromatography separates components based on their sizes.

• Gas chromatography separates components based on their shapes.

• Gas chromatography separates components based on their volatilities.

• Gas chromatography separates components based on their colors.

What are the applications of gas chromatography?

• Space exploration

• Agriculture and farming

• Pharmaceuticals, environmental analysis, food and beverage industry, and forensic science

• Fashion and clothing industry

What is the stationary phase in liquid chromatography?

• The gaseous phase used in liquid chromatography

• The phase that moves during the chromatographic process

• The mobile phase in liquid chromatography

• The solid or liquid phase that does not move during the chromatographic process.

Describe the process of high-performance liquid chromatography.

• It involves the use of a gas mobile phase and a liquid stationary phase.

• It involves the use of a liquid mobile phase and a solid stationary phase to separate the components based on their interactions with the two phases.

• It separates components based on their color rather than their interactions with the phases.

• It does not involve any mobile or stationary phase, but rather relies on temperature differences.

What are the advantages of high-performance liquid chromatography over traditional liquid chromatography?

• Advantages include improved resolution, sensitivity, and speed of analysis.

• Advantages include increased cost, complexity, and time of analysis.

• Advantages include decreased resolution, sensitivity, and speed of analysis.

• Advantages include limited application, accuracy, and efficiency of analysis.

How is the separation achieved in high-performance liquid chromatography?

• By using a magnetic field to separate the components

• By pumping the sample mixture through a column packed with stationary phase at high pressure.

• By adding a chemical reagent to the sample mixture

• By heating the sample to a high temperature

Explain the role of the mobile phase in chromatography.

• The mobile phase has no role in chromatography

• The mobile phase carries the sample through the stationary phase and helps to separate its components.

• The mobile phase is responsible for detecting the components of the sample

• The mobile phase helps to keep the stationary phase in place