biotechnology chapter 4 review (Gannon)

A carbohydrate from seaweed that is widely used as a medium for horizontal gel electrophoresis

A process that uses electricity to separate charged molecules, such as DNA fragments, RNA, and proteins, on a gel slab

• Polymerase change reaction

• Use of a microscope

• Centrifugation

• Gel electrophoresis

The acrylic box that holds the agarose gel containing the DNA samples that are analyzed when the electrophoresis process is complete

• Well comb

• Agarose

• Electrophoresis chamber

• Gel box

What is a buffer used for gel plates containing a mixture of tris base, acetic acid and EDTA

An organic compound such as sugar or starch, and is used to store energy

• Proteins

• Nucleic acids

• Carbohydrate

• Lipids

A polymer used as a gel material in vertical electrophoresis used to separate smaller molecules, like proteins and very small pieces of DNA or RNA

• Polymer

• T.A.E.

• Polyacrylamide

• Site-specific mutagenesis

Used to create the wells in gels for electrophoresis

The buffer used to fill the tank that the gel is immersed in.

The process if treating a disease or disorder by replacing a dysfunctional gene with a functional one

• Mutagen

• High through-put screening

• Site-specific mutagenesis

• Gene therapy

Large molecules composed of many similar smaller molecules linked together in a chain-like fashion

• Methylene blue

• Mutagen

• Polymer

• Lambda hind 3