Screening Transformants: Reporter Gene (GUS)

Based on the image, which order is correct (left to right)?

• SAG21 (promoter), GUS (reporter)

• GUS (promoter), SAG21 (reporter)

Which of the definitions below is correct for promoter gene?

• A genetic sequence responsible for creating new genetic materials by mixing and reshuffling existing DNA sequences within a cell, enhancing the organism's adaptability to changing environmental conditions.

• The specific DNA sequence that regulates the transcription of the gene and can activate or repress the expression of the gene.

- Gene expression: Examine the spatial and temporal expression of a gene

• - Able to visualize the overall rate of transcription from the promoter

Which gene has the general functions as described above?

• SAG21

• GUS

Full name of GUS

• α-glucaronidase

• β-glucuronidase

• α-gluconinidase

• β-glucorinidase

Full name of SAG21

• Senescence-Activation Gene 21

• Senescence-Associated Gene 21

• Synthetic Agro Growth 21

• Symbiotic Alliance Guardian 21

How does GUS gene help in detection of SAG21 expression?

• Emit fluorescence with blue light

• Accelerated senescence and chlorophyll degradation

• Hydrolyzing X-Gluc into diX indigo, a blue precipitate

• Late senescence and prolonged life span

Above image shows the process of construction of GUS gene.

• True

• False

Spatiotemporal gene expression refers to the dynamic and coordinated regulation of gene activity across both spatial and temporal dimensions within an organism.

• True

• False

Which of these descriptions are TRUE for temporal (Time-based) gene expression? (3 correct answers)

• Specific locations or regions within an organism where a particular gene is activated or suppressed.

• Regulation of gene activity over time during different stages of development, in response to environmental stimuli, or as part of various biological processes.

• Genes that play a role in embryonic development may be expressed during specific developmental stages.

• Time-course experiments & technologies like RNA sequencing

SAG21 is an Arabidopsis thaliana gene belonging to the late embryogenesis-associated (LEA) protein family that is mainly expressed in the cotyledons, roots and pollen and the expression is repressed by the light treatment in the roots. Its expression could be triggered by different stressors like drought, high salinity, or pathogen attack, yet SAG21 is not activated during senescence.

True or false for whole statement?

• True

• False