Lab Review: Isolating Gene TAS2R38 using PCR

What is the purpose of PCR amplification in isolating gene TAS2R38?

• To study the function of the TAS2R38 gene

• To remove the TAS2R38 gene

• To identify the TAS2R38 gene

• To make multiple copies of the TAS2R38 gene

What is the function of the TAS2R38 gene?

• Regulation of sweet taste

• Detection of sour taste

• Perception of bitter taste

• Perception of umami taste

Which technique is used to visualize the PCR products?

• Immunohistochemistry

• Gel electrophoresis

• Polymerase chain reaction

• Western blotting

What is the purpose of gel electrophoresis in the gene isolation process?

• To separate DNA fragments based on size and charge.

• To purify DNA samples

• To identify specific genes

• To amplify DNA fragments

Which technique is used to cut the PCR products into smaller fragments?

• DNA sequencing

• Polymerase chain reaction

• Restriction enzyme digestion

• Gel electrophoresis

What is the purpose of restriction digest in the gene isolation process?

• To amplify DNA using PCR

• To separate DNA fragments by size using gel electrophoresis

• To introduce foreign DNA into a host organism using transformation

• To cut DNA at specific sites using restriction enzymes

What is the purpose of DNA polymerase in PCR amplification?

• To amplify the RNA strands during PCR amplification.

• To stabilize the temperature during PCR amplification.

• To break down the DNA strands during PCR amplification.

• To synthesize new strands of DNA during PCR amplification.

Which step of PCR amplification involves denaturation of DNA?

• Step 1

• Step 3

• Step 4

• Step 2

During this step of PCR, primers stick to the template DNA.

• Extension

• Anneal

• Denature

There is a one base difference between the taster and nontaster alleles. This is known as a

• SNP

• Dominant Allele

• Recessive Allele

• PCR