Flow practical

What is a cell marker?

• An antibody

• A pathogen

• A machine

• A molecule expressed on/in a call

How can we target multiple markers simultaneously with traditional flow cytometry?

• By using multiple antibodies

• By using multiple antibodies bound to different fluorescent dyes

• By using electron microscopy

• By using antigens with different colours

What is a Cell Sorter?

• Flow cytometer that can identify and sort cells

• A machine that uses light absorption

• Flow cytometer that uses heavy metals as tags

• A special microscope slide

When using viability dyes, How are live cells identified in flow cytometry?

• Live cells are brighter

• Live cells are dimmer (less bright)

• Dead cells disappear

• The machine will tell you automatically

In flow cytometry, which parameter indicates the size of cells?

• Brightness of cells

• Side scatter (SSC)

• Forward scatter (FSC)

• All of the above

From the picture, What is indicated by "A"

• Lymphocytes

• Debris

• Granulocytes

• Monocytes

From the picture, What is the percentage of CD4+ cells?

• 60.9%

• 32.2%

• 62.95%

• 64.84%

From the example shown, What is the fluorescent label of CD8?

• FITC

• APC

• CD8+

• It is not shown