GC and HPLC Quiz CHEM275

How are liquid samples analyzed by gas chromatography (GC)?

• they are injected, and then the sample is carried by a liquid mobile phase

• they are vaporized after injection, and then the sample is carried by a gas mobile phase

• they are liquified after injection, and then the sample is carried by a liquid mobile phase

• they are injected, and then the sample is carried by a gas mobile phase

The time required for a component to elute out of the column is called what?

• retention time

• elution time

• column time

• reaction time

What kind of sample is preferred to be analyzed by gas chromatography (GC)?

• it must be a small (<1000 Da), nonvolatile polar molecule

• it must be a large (>1000 Da), voltatile polar molecule

• it must be a small (<1000 Da), volatile nonpolar molecule

• it must be a large (>1000 Da), nonvolatile nonpolar molecule

What are the five major components of a gas chromatography (GC) instrument?

• injection port, heating chamber, mobile phase, column, and detector

• injection port, cooling chamber, stationary phase, column, and detector

• injection port, heating chamber, stationary phase, column, and reflector

• injection port, cooling chamber, mobile phase, column, and reflector

An analyte with a (low/high) chemical affinity with the column will elute quickly through the column

(a)  

In gas chromatography (GC), the chromatogram peaks should show a(n) (asymmetrical/symmetrical) gaussian curve

(a)  

High Performance Liquid Chromatography (HPLC) is performed at a (higher/lower) flow rate and pressure than classic column chromatography

(a)  

What are the two main types of HPLC performed?

• Organic and inorganic HPLC

• Polar and nonpolar HPLC

• Simple and complex HPLC

• Analytical and preparative HPLC

The mobile phase for an HPLC often consists of:

• DI water

• Organic Solvent

• Both water and organic solvent

• Water or organic solvents, but not both

HPLC can be very versatile, and two of the most common uses for HPLC include: (select all that apply)

• Purification of peptide molecules

• Turning liquid samples into vapor

• Identification of organic compounds

• Solidifying liquid compounds