A Level Biology - Cell Fractionation & Ultracentrifugation Quiz

What is the process of separating cell organelles from each other known as?

• Filtration

• Cell Fractionation

• Homogenisation

• Ultracentrifugation

What is the purpose of homogenisation in cell fractionation?

• To separate organelles from each other

• To break up cells

• To filter out large cell debris

• To spin the mixture at different speeds

Why is the isotonic buffer solution used in homogenisation?

• To prevent organelle proteins from becoming denatured

• To prevent water from moving into the organelles via osmosis

• To separate out any large cell debris or tissue debris

• To reduce the activity of enzymes that break down organelles

What is the purpose of filtration in cell fractionation?

• To break up cells

• To separate organelles from each other

• To filter out large cell debris

• To prevent water from moving into the organelles via osmosis

What is the function of ultracentrifugation in cell fractionation?

• To filter out large cell debris

• To separate materials by spinning

• To separate organelles from each other

• To break up cells

What is the purpose of spinning the filtrate at a low speed in ultracentrifugation?

• To drain off the supernatant

• To separate out the lightest organelles

• To separate out the heaviest organelles

• To form a thick sediment known as a pellet

What is the order of mass of organelles separated out in ultracentrifugation (from heaviest to lightest)?

• Nuclei, Chloroplasts, Mitochondria, Lysosomes, Endoplasmic reticulum, Ribosomes

• Ribosomes, Endoplasmic reticulum, Lysosomes, Mitochondria, Chloroplasts, Nuclei

• Chloroplasts, Nuclei, Mitochondria, Lysosomes, Endoplasmic reticulum, Ribosomes

• Nuclei, Chloroplasts, Mitochondria, Lysosomes, Endoplasmic reticulum, Ribosomes

What is the purpose of the buffer solution in homogenisation?

• To prevent water from moving into the organelles via osmosis

• To reduce the activity of enzymes that break down organelles

• To prevent organelle proteins from becoming denatured

• To separate out any large cell debris or tissue debris

What is the function of the homogeniser in cell fractionation?

• To spin the mixture at different speeds

• To separate organelles from each other

• To break up cells

• To filter out large cell debris

What is the purpose of the gauze in filtration?

• To separate organelles from each other

• To prevent water from moving into the organelles via osmosis

• To break up cells

• To filter out large cell debris