BIOSCORE DB014 RECOMBINANT DNA TECHNOLOGY

Which of the following statement is CORRECT about recombinant DNA technology?

• is used to clone organism such as dolly the sheep

• is important in cloning genes such as growth hormone and insulin

• can be used to produce genetically engineered organism such as transgenic plant which resistant to pesticide

• primary principle is to join DNA molecules from 2 different sources forming variety of genetic recombination

The following tools are required to produce recombinant DNA, EXCEPT..

• DNA fragment containing gene of interest

• DNA ligase

• bacterial plasmid DNA

• Taq polymerase

Which are the following statements is NOT TRUE about restriction enzymes?

• Most bacterium can produce more than one type of restriction enzyme

• It cleaves DNA at a specific base sequence

• It naturally control the replication of viral DNA in bacteria for defense

• It prevent the formation of recombinant DNA in bacteria

the following figure shows sequences of bases found within plasmid and donor DNA. DNA Q were introduced with restriction enzyme to cleave open for insertion. identify the sequence of bases it recognizes.

the following figure shows sequences of bases found within plasmid and donor DNA. both DNA were introduced with restriction enzyme . name the restriction enzyme used in P.

• Sma I

• Bam HI

• EcoRI

the following figure shows sequences of bases found within plasmid and donor DNA. DNA in P were cut using restriction enzyme producing sticky ends. identify the sticky end sequence produced.

image provided shows the region within plasmid DNA which is important during screening. which of the following statement correctly describe its importance?

• marker gene contains restriction site to be cut by restriction enzyme

• marker gene is used to insert foreign DNA to produce recombinant DNA

• marker gene contains antibiotic resistant gene such as AmpR

• marker gene contains lac Z to prevent beta galactosidase synthesis

Escherichia coli is suitable to be used as host cell of the following EXCEPT

• able to undergo transformation to accept foreign DNA

• able to accept recombinant DNA by transformation

• able to ampilfy product of desired gene

• able to maintain recombinant plasmid.

the restriction site recognized by restriction enzyme contains 6 _________________ base sequence which reads the same on opposite direction on both DNA strands such as GGATCC which is recognized by BamHI enzyme.

Which of the following is NOT an example of vector?

• bacteriophage

• Escherichia coli

• plasmid DNA

• yeast artificial chromosome