Madi’s Wednesday labs

The independent variable(s) in our experiment is​/are ​

• soil not from auto shop

• soil from auto shop

• soil contaminated with motor oil

• microbial biodiversity

The dependent variable(s) in our experiment is/are​ ​ ​

• soil pH
• moisture content
• microbial diversity
• your mom
• soil texture

What is the purpose of the Ecoplate?

• to see what carbon sources are utilized in our soil sample

• to test for microbial biodiversity

• to reinforce our pipetting and teamwork

• to teach us about richness, evenness, and Shannon Index

What is the purpose of 16S rRNA sequencing?

• to determine the diversity of the microbiome in our soil samples

• to determine whether straw is helping the plants grow

• to determine what bacteria are in our soil samples

• to determine if a certain bacterium can survive in our experimental condition

Why is 16s rRNA important?

• It isn’t double stranded

• It is the most variable region in a bacteria’s DNA

• 16 is my lucky number

• Dr. Adler said it was

DOUBLE POINTS!!!! Why would soil scientists and the urban gardening community care about our experiment?

Groups:

(a) Urban Gardening

,

(b) Soil Scientists

• improve soil quality

• improve crop yield

• applicable to other studies

• microbial biodiversity

DOUBLE POINTS!!! The greatest dog ever according to Madi is