Microscopyt

Match the following

•   making an image appear larger

• Microscopy

• Clarity of object

  how clear

• magnification

• technique used to make things larger

• Resolution

Shantel wanted to look at her skin cells under a microscope. After making a wet-mount slide, she looks at it under her compound microscope. The image she sees is clear but not big enough.

• Resolution

• magnification

The power (​ (a)   ) of image = power of the ​ (b)   x power of the ​ (c)   lens.

• we use the term ​ (d)   (times 

larger) when describing the 

magnification.

• magnification
• eyepiece
• objective
• “X”

If the eyepiece has a power of 10x and the objective has a power of 30, what is the total magnification?

• 3

• 30

• 300

We can see greater detail with higher levels of magnification but we cannot see

the entire specimen.

Which has the broader field of view?

• Low power objective

• Medium power objective

• High power objective

Why does the high power objective have higher magnification, but lower field of view?

• It is closer to the stage and specimen

• It is further away from the stage and specimen

Match the following

• Light shines off specimen

• 
• Light shines through the specimen

• 
• Uses electrons to form an image

• 

How to prepare a wet mount slide

1. Get a clean slide and ​ (a)   slip.

2. Carefully place your ​ (b)   on the slide.

Place one drop of ​ (c)   If your specimen is clear or hard to see, use a stain.

Slowly lower the cover slip on top of the specimen at a ​ (d)   angle to prevent ​ (e)   bubbles.on top of your specimen.

• cover
• specimen
• water
• 45o
• air

Why do you place a slide cover on the slide at a 45 degree angle?

• To trap the organism

• To prevent air bubbles

• To trap the water