Biotechnology

What is the purpose of restriction enzymes?

• To join fragments of DNA

• To cut DNA at specific recognition sites

• To amplify DNA sequences

• To separate DNA fragments according to size

What is the function of DNA ligase?

• To join fragments of DNA

• To cut DNA at specific recognition sites

• To amplify DNA sequences

• To separate DNA fragments according to size

What is the function of a plasmid in DNA cloning?

• Amplifying DNA segments

• Cutting DNA at specific sites

• Ligating DNA fragments

• Serving as a vector to carry DNA into host cells

What is the term for the resulting DNA molecule when two different DNA fragments are joined together in DNA cloning?

• Recombinant DNA

• Replicated DNA

• Amplified DNA

• Mutated DNA

What is the purpose of bacterial transformation in DNA cloning?

• To cut and paste DNA fragments

• To amplify DNA sequences

• To join fragments of DNA

• To introduce foreign DNA into bacteria

What is the goal of PCR?

• To cut DNA at specific recognition sites

• To join fragments of DNA

• To amplify a specific DNA region

• To separate DNA fragments according to size

What is denaturation in the PCR process?

• The cooling of the reaction mixture

• The separation of DNA strands by heating

• The addition of primers to the reaction

• The final step in PCR amplification

What is the typical temperature range for the denaturation step in PCR?

• 37-42°C

• 50-60°C

• 72-75°C

• 90-95°C

What is the purpose of the extension step in PCR?

• To separate DNA strands

• To cool the reaction mixture

• To synthesize new DNA strands

• To add primers to the reaction

How many cycles of denaturation, annealing, and extension are typically performed in a PCR reaction?

• 1 cycle

• 5 cycles

• 20-40 cycles

• 90-100 cycles