Bioinformatics

Which of the following is incorrect regarding sequence homology?

• It is an important concept in sequence analysis

• When two sequences are descended from a common evolutionary origin, they are said to share homology

• Two sequences can homologous relationship even if have do not have common origin

• When two sequences are descended from a common evolutionary origin, they are said to have a homologous relationship

Your lab partner is using BLAST, and his best E value is 3. This means that...

• he’s found 3 proteins in the database that have the same sequence as his protein.

• the chance that these similarities arose due to chance is one in 10^3.

• there would be 3 matches that good in a database of this size by chance alone.

• the match in amino acid sequencs is perfect, except for the amino acids at 3 positions.

Which of the following is not a variant of BLAST?

• BLASTN

• BLASTP

• TBLASTNX

• BLASTX

In a BLAST search, the lower the E value,...

• the less amount of SNP’s and insertion/deletions were found

• The more sequence base nucleotides were used

• The more the organisms are related

• The less likely the organisms are related

Which type of skills are required to be a good bioinformatician?

Answer explanation

To be a good bioinformatician, it is necessary to have a sound knowledge of molecular biology, biochemistry, molecular biophysics, molecular modelling, computer, information technology, and biostatistics. Beside, a bioinformatics professional must understand the central dogma of molecular biology and bioinformatics software packages.

Arrange the steps of a "Basic local alignment search tool" search in the correct order:

1. 1. Specify the query sequence

   2. 2. Select "Basic local alignment search tool" program

3. 3. Check total score, max score and e -value

4. 4. Conduct the "Basic local alignment search tool" search

5. 5. Select a database

What is GenBank?

What is a biological database in bioinformatics?

Answer explanation

database used to store and manage biological data and information in computer-readable forms

What is the Basic Local Alignment Search Tool (BLAST)?

Answer explanation

A tool used for comparing sequence information and retrieving sequences from databases

Informatics in Bioinformatics can perform

• Data Mining

• Data Management

• Data Computation

• All of these