Basic Molecular Diagnostics

A process wherein DNA is "converted" to RNA.

• Replication

• Transcription

• Conversion

• Translation

TRUE OR FALSE

1. 1. Proteins are composed of amino acids which are attached to one another in long chains.

2. 2. A sequence of three nucleotides which form a unit of genetic code in the DNA/RNA is called codons.

• Statement 1 is true. Statement 2 is false.

• Statement 1 is false. Statement 2 is true.

• Both statements 1 and 2 are true.

• Both statements 1 and 2 are false.

All of the following DNA base pairings are correct, EXCEPT:

• Adenine - Thymine

• Cytosine - Guanine

• Adenine - Uracil

• Thymine - Guanine

Which of the following nucleotide is/are seen in RNA?

• Adenine

• Thymine

• Uracil

• Cytosine

What type of mutation?

ACAAGC - ACAGC

• SNP

• Substitution

• Insertion

• Deletion

What type of mutation?

GCGGAC - GGCGGAC

• SNP

• Substitution

• Insertion

• Deletion

Which of the following is a start codon?

• UAA

• UAG

• AUG

• AUA

Which of the following PCR is being employed in TrueNat technology?

• Conventional PCR

• Real-time PCR

• Reverse-transcription PCR

• Nested PCR

This is an enzyme that elongates primers based on the DNA template.

• Polymerase

• Ligase

• Nuclease

• None of the above.

Arrange the following steps of PCR processing correctly.

1. 1. Annealing

2. 2. Denaturation

3. 3. Elongation

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