Transgenic Plants 1

Another name for Agrobacterium tumefaciens is

• Rhizobium radiobacter
• Rhizobium radiodurens
• Agrobacterium rhizogenes
• Agrobacterium radiobacter

Label the given diagram of the T-DNA of a Ti plasmid.

c

d

e

aux

LB

rolA

ocs

ori

vir

Which of these are needed for agrobacterium mediated transformation of a plant with a transgene?

• vir

• Left and right borders

• aux and cyt

• ori

• ocs

A. tumefaciens grows optimally at which temperature?

• 28 °C
• 30 °C
• 37 °C
• 12 °C

chvE protein increases ____ protein sensitivity to phenolic compounds.

• Vir A
• Vir E
• Vir D
• Vir G

Arrange in correct order the steps involved in infection of a plant by A. tumeficiansReorder the following

• VirA phosphorylates VirG activating it

• A. tumeficians senses phenolics and is attracted to plant

• Phenolics bind to the bacterial transmembrane protein VirA

• VirD1 and VirD2 are expressed

Which Vir proteins contains a Nuclear localization signal to import T-DNA into the plant cell nucleus?

• Vir E2 and Vir D2
• Vir E1 and Vir D1
• Vir D1 and Vir D2
• Vir E1 and Vir E2

What are nptII and aphII?

• Antibiotic resistance markers
• Herbicide resistance markers
• Nutritional inhibitor marker
• Reporter genes

Agrobacterium rhizogenes can be used to transform which type of host plant?

• Only eudicots
• Only monocots
• Both eudicots and monocots

Hairy root formation is seen in an A. rhizogenes infected plant because...

• It infects only the root cells of plants
• It sensitizes the plant to endogenous auxins
• It contains genes that increase synthesis of auxins
• It requires more nutrients from the soil to grow