BMS1062 week 5 content

1. Which of the following is true of RNA synthesis (transcription)?

• RNA synthesis is always in the 3' - 5' direction

• It is carried out by RNA polymerase

• In transcription, U is inserted opposite T

• New nucleotides are added on to the 5' OH of the ribose sugar

If a coding sequence reads 5’ ATGTCG 3’, what will the RNA produced be?

• 5’ AUGUCG 3’

• 5’ GCUGUA 3’

• 5’ UACAGC 3’

• 5’ CGACAU 3’

Which of the following is true regarding transcription in prokaryotes?

• Prokaryotes have 3 types of RNA polymerase

• Transcription begins with RNA polymerase binding to the enhancer

• Rho-dependent termination involves the RUT sequence

• Intrinsic termination occurs at AT-rich areas followed by string of U’s

Which of the following is false? The genetic code:

• Uses nucleotide triplets called codons

• Is overlapping with 3 reading frames

• Is degenerate

• Is unambiguous

Which of the following is true about codons?

• There are 61 codons

• UAG, UAA and UGA are stop codons

• AUG codes for f-methionine only

• One codon can encode many amino acids

Which is not involved in translation?

• Ribosome

• tRNA

• Aminoacyl-mRNA synthetase

• mRNA

What does the RNA polymerase holoenzyme do?

• It helps activate RNA polymerase

• It helps terminate RNA synthesis

• It locates the promoter on the DNA

• It is the catalytic centre of RNA polymerase

Which of the following are features of the wobble hypothesis?

• A tRNA can recognise only one codon

• Some tRNAs can recognise codons that specify two different amino acids if both are nonpolar

• The “wobble” only occurs in the first base of the codon

• The “wobble” only occurs in the third base of the codon

Which of the following is true?

• Many molecules of RNA polymerase simultaneously transcribe one gene

• Promoters of E.coli genes contain one strongly conserved region

• The energy for RNA synthesis comes from the hydrolysis of ATP

• Both the template strand and coding strand can be transcribed at any one time